MS Data Miner: a web-based software tool to analyze, compare, and share mass spectrometry protein identifications.

Published

Journal Article

Data processing and analysis of proteomics data are challenging and time consuming. In this paper, we present MS Data Miner (MDM) (http://sourceforge.net/p/msdataminer), a freely available web-based software solution aimed at minimizing the time required for the analysis, validation, data comparison, and presentation of data files generated in MS software, including Mascot (Matrix Science), Mascot Distiller (Matrix Science), and ProteinPilot (AB Sciex). The program was developed to significantly decrease the time required to process large proteomic data sets for publication. This open sourced system includes a spectra validation system and an automatic screenshot generation tool for Mascot-assigned spectra. In addition, a Gene Ontology term analysis function and a tool for generating comparative Excel data reports are included. We illustrate the benefits of MDM during a proteomics study comprised of more than 200 LC-MS/MS analyses recorded on an AB Sciex TripleTOF 5600, identifying more than 3000 unique proteins and 3.5 million peptides.

Full Text

Cited Authors

  • Dyrlund, TF; Poulsen, ET; Scavenius, C; Sanggaard, KW; Enghild, JJ

Published Date

  • September 2012

Published In

Volume / Issue

  • 12 / 18

Start / End Page

  • 2792 - 2796

PubMed ID

  • 22833312

Pubmed Central ID

  • 22833312

Electronic International Standard Serial Number (EISSN)

  • 1615-9861

International Standard Serial Number (ISSN)

  • 1615-9853

Digital Object Identifier (DOI)

  • 10.1002/pmic.201200109

Language

  • eng