NMR reveals two-step association of Congo Red to amyloid β in low-molecular-weight aggregates.

Published

Journal Article

Aggregation of the Amyloid β peptide into amyloid fibrils is closely related to development of Alzheimer's disease. Many small aromatic compounds have been found to act as inhibitors of fibril formation, and have inspired the search for new drug candidates. However, the detailed mechanisms of inhibition are largely unknown. In this study, we have examined in detail the binding of the fibril-formation inhibitor Congo Red (CR) to monomeric Aβ(1-40) using a combination of 1D, 2D, saturation transfer difference, and diffusion NMR, as well as dynamic light scattering experiments. Our results show that CR binds to the fibril forming stretches of Aβ(1-40) monomers, and that complex formation occurs in two steps: An initial 1:1 CR:Aβ(1-40) complex is formed by a relatively strong interaction (K(d) ≈ 5 μM), and a 2:1 complex is formed by binding another CR molecule in a subsequent weaker binding step (K(d) ≈ 300 μM). The size of these complexes is comparable to that of Aβ(1-40) alone. The existence of two different complexes might explain the contradictory reports regarding the inhibitory effects of CR on the fibril-formation process.

Full Text

Cited Authors

  • Pedersen, MØ; Mikkelsen, K; Behrens, MA; Pedersen, JS; Enghild, JJ; Skrydstrup, T; Malmendal, A; Nielsen, NC

Published Date

  • December 2010

Published In

Volume / Issue

  • 114 / 48

Start / End Page

  • 16003 - 16010

PubMed ID

  • 21077638

Pubmed Central ID

  • 21077638

Electronic International Standard Serial Number (EISSN)

  • 1520-5207

International Standard Serial Number (ISSN)

  • 1520-6106

Digital Object Identifier (DOI)

  • 10.1021/jp108035y

Language

  • eng