The TSG-6/HC2-mediated transfer is a dynamic process shuffling heavy chains between glycosaminoglycans.


Journal Article

The heavy chain (HC) subunits of the bikunin proteins are covalently attached to a single chondroitin sulfate (CS) chain originating from bikunin and can be transferred to different hyaluronan (HA) molecules by TSG-6/HC2. In the present study, we demonstrate that HCs transferred to HA may function as HC donors in subsequent transfer reactions, and we show that the CS of bikunin may serve as an HC acceptor, analogous to HA. Our data suggest that TSG-6/HC2 link HCs randomly on the CS chain of bikunin, in contrast to the ordered attachment observed during the biosynthesis. Moreover, the results show that the transfer activity is indifferent to the new HC position, and the relocated HCs are thus prone to further TSG-6/HC2-induced transfer reactions. The data suggest that HCs may be transferred directly from HA to HA without the involvement of the bikunin CS chain. The results demonstrate reversibility of the interactions between HCs and glycosaminoglycans and suggest that a dynamic shuffling of the HCs occur in vivo.

Full Text

Cited Authors

  • Sanggaard, KW; Scavenius, C; Rasmussen, AJ; Wisniewski, H-G; Thøgersen, IB; Enghild, JJ

Published Date

  • July 2010

Published In

Volume / Issue

  • 285 / 29

Start / End Page

  • 21988 - 21993

PubMed ID

  • 20463016

Pubmed Central ID

  • 20463016

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

International Standard Serial Number (ISSN)

  • 1083-351X

Digital Object Identifier (DOI)

  • 10.1074/jbc.M109.041046


  • eng