Large scale isolation and purification of soluble RAGE from lung tissue.


Journal Article

The receptor for advanced glycation end-products (RAGE) has been implicated in numerous disease processes including: atherosclerosis, diabetic nephropathy, impaired wound healing and neuropathy to name a few. Treatment of animals with a soluble isoform of the receptor (sRAGE) has been shown to prevent and even reverse many disease processes. Isolating large quantities of pure sRAGE for in vitro and in vivo studies has hindered its development as a therapeutic strategy in other RAGE mediated diseases that require long-term therapy. This article provides an improvement in both yield and detail of a previously published method to obtain 10mg of pure, endotoxin free sRAGE from 65 g of lung tissue.

Full Text

Cited Authors

  • Englert, JM; Ramsgaard, L; Valnickova, Z; Enghild, JJ; Oury, TD

Published Date

  • September 2008

Published In

Volume / Issue

  • 61 / 1

Start / End Page

  • 99 - 101

PubMed ID

  • 18558495

Pubmed Central ID

  • 18558495

Electronic International Standard Serial Number (EISSN)

  • 1096-0279

International Standard Serial Number (ISSN)

  • 1046-5928

Digital Object Identifier (DOI)

  • 10.1016/j.pep.2008.05.004


  • eng