The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2.


Journal Article

Tumor necrosis factor-stimulated gene-6 protein (TSG-6) is involved in the transfer of heavy chains (HCs) from inter-alpha-inhibitor (IalphaI), pre-alpha-inhibitor, and as shown here HC2.bikunin to hyaluronan through the formation of covalent HC.TSG-6 intermediates. In contrast to IalphaI and HC2.bikunin, pre-alpha-inhibitor does not form a covalent complex in vitro using purified proteins but needs the presence of another factor (Rugg, M. S., Willis, A. C., Mukhopadhyay, D., Hascall, V. C., Fries, E., Fülöp, C., Milner, C. M., and Day, A. J. (2005) J. Biol. Chem. 280, 25674-25686). In the present study we purified the required component from human plasma and identified it as HC2. Proteins containing HC2 including IalphaI, HC2.bikunin, and free HC2 promoted the formation of HC3.TSG-6 and subsequently HC3.hyaluronan complexes. HC1 or HC3 did not possess this activity. The presented data reveal that both HC2 and TSG-6 are required for the transesterification reactions to occur.

Full Text

Cited Authors

  • Sanggaard, KW; Sonne-Schmidt, CS; Krogager, TP; Lorentzen, KA; Wisniewski, H-G; Thøgersen, IB; Enghild, JJ

Published Date

  • July 2008

Published In

Volume / Issue

  • 283 / 27

Start / End Page

  • 18530 - 18537

PubMed ID

  • 18448433

Pubmed Central ID

  • 18448433

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.m800874200


  • eng