Purification and characterization of mouse soluble receptor for advanced glycation end products (sRAGE).


Journal Article

The receptor for advanced glycation end products (RAGE) is a member of the immunoglobulin superfamily of cell surface proteins that has been implicated as a progression factor in a number of pathologic conditions from chronic inflammation to cancer to Alzheimer's disease. In such conditions, RAGE acts to facilitate pathogenic processes. Its secreted isoform, soluble RAGE or sRAGE, has the ability to prevent RAGE signaling by acting as a decoy. sRAGE has been used successfully in animal models of a range of diseases to antagonize RAGE-mediated pathologic processes. In humans, sRAGE results from alternative splicing of RAGE mRNA. This study was aimed to determine whether the same holds true for mouse sRAGE and, in addition, to biochemically characterize mouse sRAGE. The biochemical characteristics examined include glycosylation and disulfide patterns. In addition, sRAGE was found to bind heparin, which may mediate its distribution in the extracellular matrix and cell surfaces of tissues. Finally, our data indicated that sRAGE in the mouse is likely produced by carboxyl-terminal truncation, in contrast to the alternative splicing mechanism reported in humans.

Full Text

Cited Authors

  • Hanford, LE; Enghild, JJ; Valnickova, Z; Petersen, SV; Schaefer, LM; Schaefer, TM; Reinhart, TA; Oury, TD

Published Date

  • November 2004

Published In

Volume / Issue

  • 279 / 48

Start / End Page

  • 50019 - 50024

PubMed ID

  • 15381690

Pubmed Central ID

  • 15381690

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

International Standard Serial Number (ISSN)

  • 1083-351X

Digital Object Identifier (DOI)

  • 10.1074/jbc.M409782200


  • eng