Dunning rat prostate adenocarcinomas and alternative splicing reporters: powerful tools to study epithelial plasticity in prostate tumors in vivo.
Journal Article (Journal Article)
Using alternative splicing reporters we have previously observed mesenchymal epithelial transitions in Dunning AT3 rat prostate tumors. We demonstrate here that the Dunning DT and AT3 cells, which express epithelial and mesenchymal markers, respectively, represent an excellent model to study epithelial transitions since these cells recapitulate gene expression profiles observed during human prostate cancer progression. In this manuscript we also present the development of two new tools to study the epithelial transitions by imaging alternative splicing decisions: a bichromatic fluorescence reporter to evaluate epithelial transitions in culture and in vivo, and a luciferase reporter to visualize the distribution of mesenchymal epithelial transitions in vivo.
Full Text
Duke Authors
Cited Authors
- Oltean, S; Febbo, PG; Garcia-Blanco, MA
Published Date
- 2008
Published In
Volume / Issue
- 25 / 6
Start / End Page
- 611 - 619
PubMed ID
- 18523850
Pubmed Central ID
- PMC2471395
International Standard Serial Number (ISSN)
- 0262-0898
Digital Object Identifier (DOI)
- 10.1007/s10585-008-9186-y
Language
- eng
Conference Location
- Netherlands