Comparison of different drying procedures for scanning electron microscopy using human leukocytes.


Journal Article

Using human leukocytes as test specimens, three different drying procedures for scanning electron microscopy: critical-point drying (CPD), Peldri II, and tetramethylsilane (TMS), were compared. All three procedures produced identical surface morphology preservation. An equal amount of volume shrinkage was observed regardless of the dehydrants and drying techniques employed. Considering the simplicity, convenience, and time saved, air-drying with TMS is by far the best choice for preparing animal cells for scanning electron microscopy.

Full Text

Duke Authors

Cited Authors

  • Ting-Beall, HP; Zhelev, DV; Hochmuth, RM

Published Date

  • November 1995

Published In

Volume / Issue

  • 32 / 4

Start / End Page

  • 357 - 361

PubMed ID

  • 8573784

Pubmed Central ID

  • 8573784

Electronic International Standard Serial Number (EISSN)

  • 1097-0029

International Standard Serial Number (ISSN)

  • 1059-910X

Digital Object Identifier (DOI)

  • 10.1002/jemt.1070320409


  • eng