Cloning and physical mapping of the cysB region of Salmonella typhimurium.

Published

Journal Article

The cysB region of Salmonella typhimurium was cloned in pBR322 and localized to a 1.75-kilobase HincII fragment. Two-dimensional protein electropherograms showed levels of the cysB polypeptide chain that were several fold higher in plasmid-bearing strains than in the wild type. Fully derepressed levels of sulfite reductase and O-acetylserine sulfhydrylase in cysB plasmid-bearing strains were only 25% higher than in the wild type, suggesting that the product of this regulatory gene ordinarily is not a limiting factor in the expression of the cysteine regulon. The mapping of cysB deletions by Southern blots showed a good correlation between the genetic and the physical maps of this gene. The supX gene was initially cloned with cysB and is within 0.7 kilobase of cysB.

Full Text

Cited Authors

  • Jagura-Burdzy, G; Kredich, NM

Published Date

  • August 1983

Published In

Volume / Issue

  • 155 / 2

Start / End Page

  • 578 - 585

PubMed ID

  • 6307971

Pubmed Central ID

  • 6307971

International Standard Serial Number (ISSN)

  • 0021-9193

Language

  • eng

Conference Location

  • United States