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Programming human dendritic cells with mRNA.

Publication ,  Journal Article
Lee, J; Boczkowski, D; Nair, S
Published in: Methods Mol Biol
2013

Transfecting with in vitro transcribed, protein-encoding mRNA is a simple yet effective method to express high levels of the desired RNA-encoded proteins in primary cells. Cells can be transfected with antigen-encoding mRNA, which is translated into protein and is processed by the cellular antigen-processing pathway to generate antigen-presenting cells. Another elegant and increasingly popular application is to transfect cells with mRNA that encodes immune modulating molecules (cytokines, chemokines, toll-like receptors (TLRs), immune receptor ligands, immune receptor targeting antibodies) which, when translated into protein, can program cell behavior and/or function. In this chapter we describe an efficient method to deliver mRNA into human dendritic cells (DCs) by electroporation. This is currently the method of choice to deliver mRNA into antigen-presenting cells for generating vaccines for cancer immunotherapy.

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Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

969

Start / End Page

111 / 125

Location

United States

Related Subject Headings

  • Transfection
  • Toll-Like Receptors
  • RNA, Messenger
  • Neoplasms
  • Humans
  • Gene Expression
  • Developmental Biology
  • Dendritic Cells
  • Cytokines
  • Cell Line
 

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Lee, J., Boczkowski, D., & Nair, S. (2013). Programming human dendritic cells with mRNA. Methods Mol Biol, 969, 111–125. https://doi.org/10.1007/978-1-62703-260-5_8
Lee, Jaewoo, David Boczkowski, and Smita Nair. “Programming human dendritic cells with mRNA.Methods Mol Biol 969 (2013): 111–25. https://doi.org/10.1007/978-1-62703-260-5_8.
Lee J, Boczkowski D, Nair S. Programming human dendritic cells with mRNA. Methods Mol Biol. 2013;969:111–25.
Lee, Jaewoo, et al. “Programming human dendritic cells with mRNA.Methods Mol Biol, vol. 969, 2013, pp. 111–25. Pubmed, doi:10.1007/978-1-62703-260-5_8.
Lee J, Boczkowski D, Nair S. Programming human dendritic cells with mRNA. Methods Mol Biol. 2013;969:111–125.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2013

Volume

969

Start / End Page

111 / 125

Location

United States

Related Subject Headings

  • Transfection
  • Toll-Like Receptors
  • RNA, Messenger
  • Neoplasms
  • Humans
  • Gene Expression
  • Developmental Biology
  • Dendritic Cells
  • Cytokines
  • Cell Line