Engineering B cells with mRNA.

Journal Article (Journal Article;Review)

Ex vivo activated B cells are an alternative source of antigen presenting cells (APC). However, the ability of ex vivo activated B cells to function as potent APCs has been a concern especially when compared to dendritic cells (DC). Herein, we introduce a strategy to modulate antigen presentation and immune stimulation functions of activated B cells by co-transfection with multiple mRNAs encoding costimulatory molecules (OX40L, 4-1BBL, and CD80), cytokines (IL-12p35 and IL-12p40) and antigen. These activated B cells modified to express immune stimulatory molecules can be a potent alternative to DCs in immunotherapy.

Full Text

Duke Authors

Cited Authors

  • Lee, J; Boczkowski, D; Nair, S

Published Date

  • 2013

Published In

Volume / Issue

  • 969 /

Start / End Page

  • 101 - 110

PubMed ID

  • 23296930

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-62703-260-5_7


  • eng

Conference Location

  • United States