Engineering B cells with mRNA.
Publication
, Journal Article
Lee, J; Boczkowski, D; Nair, S
Published in: Methods Mol Biol
2013
Ex vivo activated B cells are an alternative source of antigen presenting cells (APC). However, the ability of ex vivo activated B cells to function as potent APCs has been a concern especially when compared to dendritic cells (DC). Herein, we introduce a strategy to modulate antigen presentation and immune stimulation functions of activated B cells by co-transfection with multiple mRNAs encoding costimulatory molecules (OX40L, 4-1BBL, and CD80), cytokines (IL-12p35 and IL-12p40) and antigen. These activated B cells modified to express immune stimulatory molecules can be a potent alternative to DCs in immunotherapy.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
969
Start / End Page
101 / 110
Location
United States
Related Subject Headings
- Transfection
- RNA, Messenger
- Lymphocyte Activation
- Interleukin-12 Subunit p40
- Interleukin-12 Subunit p35
- Humans
- Developmental Biology
- Cell Engineering
- B-Lymphocytes
- Antigens, CD
Citation
APA
Chicago
ICMJE
MLA
NLM
Lee, J., Boczkowski, D., & Nair, S. (2013). Engineering B cells with mRNA. Methods Mol Biol, 969, 101–110. https://doi.org/10.1007/978-1-62703-260-5_7
Lee, Jaewoo, David Boczkowski, and Smita Nair. “Engineering B cells with mRNA.” Methods Mol Biol 969 (2013): 101–10. https://doi.org/10.1007/978-1-62703-260-5_7.
Lee J, Boczkowski D, Nair S. Engineering B cells with mRNA. Methods Mol Biol. 2013;969:101–10.
Lee, Jaewoo, et al. “Engineering B cells with mRNA.” Methods Mol Biol, vol. 969, 2013, pp. 101–10. Pubmed, doi:10.1007/978-1-62703-260-5_7.
Lee J, Boczkowski D, Nair S. Engineering B cells with mRNA. Methods Mol Biol. 2013;969:101–110.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2013
Volume
969
Start / End Page
101 / 110
Location
United States
Related Subject Headings
- Transfection
- RNA, Messenger
- Lymphocyte Activation
- Interleukin-12 Subunit p40
- Interleukin-12 Subunit p35
- Humans
- Developmental Biology
- Cell Engineering
- B-Lymphocytes
- Antigens, CD