Cytological evidence for DNA chain elongation after UV irradiation in the S phase.

Published

Journal Article

Human cells irradiated with UV light synthesize lower molecular weight DNA than unirradiated cells. This reduction in molecular weight is greater in xeroderma pigmentosum (XP) cells than in normal cells. The molecular weight of DNA is further reduced by the addition of caffeine to XP cells. By several hours after irradiation, DNA fragments are barely detectable. Cells from excision-proficient and excision-deficient XP patients were studied autoradiographically to produce cytological evidence of DNA chain elongation. Replicate cultures with and without caffeine were synchronized and irradiated with UV light during the S phase. Caffeine was removed in G2, and the cells were labeled with 3H-thymidine. Results showed significantly increased labeling during G2 of excision-deficient XP cells. Labeling was dependent on the time of irradiation and presence of caffeine. The XP variant cells had no increase in labeling for any irradiation time.

Full Text

Cited Authors

  • Minka, DF; Nath, J

Published Date

  • April 1, 1981

Published In

Volume / Issue

  • 19 / 3-4

Start / End Page

  • 199 - 210

PubMed ID

  • 7247930

Pubmed Central ID

  • 7247930

Electronic International Standard Serial Number (EISSN)

  • 1573-4927

International Standard Serial Number (ISSN)

  • 0006-2928

Digital Object Identifier (DOI)

  • 10.1007/bf00504267

Language

  • eng