Genome-wide chromatin state transitions associated with developmental and environmental cues.

Journal Article (Journal Article)

Differences in chromatin organization are key to the multiplicity of cell states that arise from a single genetic background, yet the landscapes of in vivo tissues remain largely uncharted. Here, we mapped chromatin genome-wide in a large and diverse collection of human tissues and stem cells. The maps yield unprecedented annotations of functional genomic elements and their regulation across developmental stages, lineages, and cellular environments. They also reveal global features of the epigenome, related to nuclear architecture, that also vary across cellular phenotypes. Specifically, developmental specification is accompanied by progressive chromatin restriction as the default state transitions from dynamic remodeling to generalized compaction. Exposure to serum in vitro triggers a distinct transition that involves de novo establishment of domains with features of constitutive heterochromatin. We describe how these global chromatin state transitions relate to chromosome and nuclear architecture, and discuss their implications for lineage fidelity, cellular senescence, and reprogramming.

Full Text

Duke Authors

Cited Authors

  • Zhu, J; Adli, M; Zou, JY; Verstappen, G; Coyne, M; Zhang, X; Durham, T; Miri, M; Deshpande, V; De Jager, PL; Bennett, DA; Houmard, JA; Muoio, DM; Onder, TT; Camahort, R; Cowan, CA; Meissner, A; Epstein, CB; Shoresh, N; Bernstein, BE

Published Date

  • January 31, 2013

Published In

Volume / Issue

  • 152 / 3

Start / End Page

  • 642 - 654

PubMed ID

  • 23333102

Pubmed Central ID

  • PMC3563935

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2012.12.033


  • eng

Conference Location

  • United States