Bacterial flavohemoglobin: a molecular tool to probe mammalian nitric oxide biology.

Journal Article (Journal Article)

A wide range of mammalian signaling and stress pathways are mediated by nitric oxide (NO), which is synthesized in vivo by the nitric oxide synthase (NOS) family of enzymes. Experimental manipulations of NO are frequently achieved by either inhibition or activation of endogenous NOS or via providing exogenous NO sources. On the contrary, many microbes consume NO via flavohemoglobin (FlavoHb), a highly efficient NO-dioxygenase that protects from nitrosative stress. Here we report a novel resource for studying NO in mammalian cells by heterologously expressing Escherichia coli FlavoHb within a lentiviral delivery system. This technique boosts endogenous cellular consumption of NO, thus providing a simple and efficacious approach to studying mammalian NO biology that can be employed as both a primary experimental and confirmatory tool.

Full Text

Duke Authors

Cited Authors

  • Forrester, MT; Eyler, CE; Rich, JN

Published Date

  • January 2011

Published In

Volume / Issue

  • 50 / 1

Start / End Page

  • 41 - 45

PubMed ID

  • 21231921

Pubmed Central ID

  • PMC3096140

Electronic International Standard Serial Number (EISSN)

  • 1940-9818

Digital Object Identifier (DOI)

  • 10.2144/000113586


  • eng

Conference Location

  • England