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Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent.

Publication ,  Journal Article
Mangel, AW; Prpic, V; Wong, H; Basavappa, S; Hurst, LJ; Scott, L; Garman, RL; Hayes, JS; Sharara, AI; Snow, ND
Published in: Am J Physiol
January 1995

The secretion of cholecystokinin was examined in STC-1 cells, an intestinal cholecystokinin (CCK)-secreting cell line. Exposure to the amino acid L-phenylalanine increased release of CCK by 135%, 180%, and 251% of control levels after 15-min treatments with 5, 20, and 50 mM phenylalanine, respectively. L-Phenylalanine-induced secretion of CCK was inhibited by the calcium channel blocker diltiazem (10 microM). L-Phenylalanine (20 mM) also significantly increased cytosolic calcium levels in fura 2-acetoxymethyl ester (fura 2-AM)-loaded cells, and this increase was diltiazem sensitive. D-Phenylalanine, over the dose range of 5-50 mM, produced nonsignificant increases in CCK release. Treatment of STC-1 cells with 300 ng/ml of pertussis toxin for either 4 or 24 h did not significantly affect either basal release of CCK or L-phenylalanine-stimulated secretion. Patch-clamp recordings from cell-attached membrane patches showed a stimulation in calcium channel activity after L-phenylalanine. These results indicate that, in STC-1 cells, L-phenylalanine stimulates release of cholecystokinin via a calcium-dependent process.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

January 1995

Volume

268

Issue

1 Pt 1

Start / End Page

G90 / G94

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Phenylalanine
  • Mice
  • GTP-Binding Proteins
  • Fluorescence
  • Electrophysiology
  • Diltiazem
  • Cytosol
  • Cholecystokinin
  • Cardiovascular System & Hematology
 

Citation

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ICMJE
MLA
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Mangel, A. W., Prpic, V., Wong, H., Basavappa, S., Hurst, L. J., Scott, L., … Snow, N. D. (1995). Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent. Am J Physiol, 268(1 Pt 1), G90–G94. https://doi.org/10.1152/ajpgi.1995.268.1.G90
Mangel, A. W., V. Prpic, H. Wong, S. Basavappa, L. J. Hurst, L. Scott, R. L. Garman, J. S. Hayes, A. I. Sharara, and N. D. Snow. “Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent.Am J Physiol 268, no. 1 Pt 1 (January 1995): G90–94. https://doi.org/10.1152/ajpgi.1995.268.1.G90.
Mangel AW, Prpic V, Wong H, Basavappa S, Hurst LJ, Scott L, et al. Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent. Am J Physiol. 1995 Jan;268(1 Pt 1):G90–4.
Mangel, A. W., et al. “Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent.Am J Physiol, vol. 268, no. 1 Pt 1, Jan. 1995, pp. G90–94. Pubmed, doi:10.1152/ajpgi.1995.268.1.G90.
Mangel AW, Prpic V, Wong H, Basavappa S, Hurst LJ, Scott L, Garman RL, Hayes JS, Sharara AI, Snow ND. Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent. Am J Physiol. 1995 Jan;268(1 Pt 1):G90–G94.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

January 1995

Volume

268

Issue

1 Pt 1

Start / End Page

G90 / G94

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Phenylalanine
  • Mice
  • GTP-Binding Proteins
  • Fluorescence
  • Electrophysiology
  • Diltiazem
  • Cytosol
  • Cholecystokinin
  • Cardiovascular System & Hematology