Phenylalanine-stimulated secretion of cholecystokinin is calcium dependent.

Journal Article (Journal Article)

The secretion of cholecystokinin was examined in STC-1 cells, an intestinal cholecystokinin (CCK)-secreting cell line. Exposure to the amino acid L-phenylalanine increased release of CCK by 135%, 180%, and 251% of control levels after 15-min treatments with 5, 20, and 50 mM phenylalanine, respectively. L-Phenylalanine-induced secretion of CCK was inhibited by the calcium channel blocker diltiazem (10 microM). L-Phenylalanine (20 mM) also significantly increased cytosolic calcium levels in fura 2-acetoxymethyl ester (fura 2-AM)-loaded cells, and this increase was diltiazem sensitive. D-Phenylalanine, over the dose range of 5-50 mM, produced nonsignificant increases in CCK release. Treatment of STC-1 cells with 300 ng/ml of pertussis toxin for either 4 or 24 h did not significantly affect either basal release of CCK or L-phenylalanine-stimulated secretion. Patch-clamp recordings from cell-attached membrane patches showed a stimulation in calcium channel activity after L-phenylalanine. These results indicate that, in STC-1 cells, L-phenylalanine stimulates release of cholecystokinin via a calcium-dependent process.

Full Text

Duke Authors

Cited Authors

  • Mangel, AW; Prpic, V; Wong, H; Basavappa, S; Hurst, LJ; Scott, L; Garman, RL; Hayes, JS; Sharara, AI; Snow, ND

Published Date

  • January 1995

Published In

Volume / Issue

  • 268 / 1 Pt 1

Start / End Page

  • G90 - G94

PubMed ID

  • 7840211

International Standard Serial Number (ISSN)

  • 0002-9513

Digital Object Identifier (DOI)

  • 10.1152/ajpgi.1995.268.1.G90


  • eng

Conference Location

  • United States