Endogenous protein S-Nitrosylation in E. coli: regulation by OxyR.

Journal Article

Endogenous S-nitrosylation of proteins, a principal mechanism of cellular signaling in eukaryotes, has not been observed in microbes. We report that protein S-nitrosylation is an obligate concomitant of anaerobic respiration on nitrate in Escherichia coli. Endogenous S-nitrosylation during anaerobic respiration is controlled by the transcription factor OxyR, previously thought to operate only under aerobic conditions. Deletion of OxyR resulted in large increases in protein S-nitrosylation, and S-nitrosylation of OxyR induced transcription from a regulon that is distinct from the regulon induced by OxyR oxidation. Furthermore, products unique to the anaerobic regulon protected against S-nitrosothiols, and anaerobic growth of E. coli lacking OxyR was impaired on nitrate. Thus, OxyR serves as a master regulator of S-nitrosylation, and alternative posttranslational modifications of OxyR control distinct transcriptional responses.

Full Text

Duke Authors

Cited Authors

  • Seth, D; Hausladen, A; Wang, Y-J; Stamler, JS

Published Date

  • April 27, 2012

Published In

Volume / Issue

  • 336 / 6080

Start / End Page

  • 470 - 473

PubMed ID

  • 22539721

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.1215643

Language

  • eng

Conference Location

  • United States