Endothelial type nitric oxide synthase in skeletal muscle fibers: mitochondrial relationships.

Journal Article (Journal Article)

Immunohistochemistry shows that skeletal muscle fibers express endothelial-type (ec)-NOS in a heterogeneous pattern distinct from that of neuronal (nc)-NOS. Analysis of adjacent serial sections reveals: 1) a strong correlation of ec-NOS expression to mitochondrial content (visualized histochemically by succinate dehydrogenase); 2) lack of correlation of ec-NOS to fiber ATPase type (in contrast to nc-NOS); and 3) constitutive co-expression of ec- and nc-NOS in some fibers. Preparations of mitochondria from diaphragm exhibited calcium-dependent NOS activity, which functioned to inhibit in vitro oxygen consumption. The results establish that normal skeletal muscle cells can express two constitutive isoforms of NOS (nc- and ec-) and suggest a functional role for ec-NOS in oxidative, mitochondria-rich fibers.

Full Text

Duke Authors

Cited Authors

  • Kobzik, L; Stringer, B; Balligand, JL; Reid, MB; Stamler, JS

Published Date

  • June 15, 1995

Published In

Volume / Issue

  • 211 / 2

Start / End Page

  • 375 - 381

PubMed ID

  • 7540837

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.1995.1824


  • eng

Conference Location

  • United States