Endothelial type nitric oxide synthase in skeletal muscle fibers: mitochondrial relationships.
Published
Journal Article
Immunohistochemistry shows that skeletal muscle fibers express endothelial-type (ec)-NOS in a heterogeneous pattern distinct from that of neuronal (nc)-NOS. Analysis of adjacent serial sections reveals: 1) a strong correlation of ec-NOS expression to mitochondrial content (visualized histochemically by succinate dehydrogenase); 2) lack of correlation of ec-NOS to fiber ATPase type (in contrast to nc-NOS); and 3) constitutive co-expression of ec- and nc-NOS in some fibers. Preparations of mitochondria from diaphragm exhibited calcium-dependent NOS activity, which functioned to inhibit in vitro oxygen consumption. The results establish that normal skeletal muscle cells can express two constitutive isoforms of NOS (nc- and ec-) and suggest a functional role for ec-NOS in oxidative, mitochondria-rich fibers.
Full Text
Duke Authors
Cited Authors
- Kobzik, L; Stringer, B; Balligand, JL; Reid, MB; Stamler, JS
Published Date
- June 15, 1995
Published In
Volume / Issue
- 211 / 2
Start / End Page
- 375 - 381
PubMed ID
- 7540837
Pubmed Central ID
- 7540837
International Standard Serial Number (ISSN)
- 0006-291X
Digital Object Identifier (DOI)
- 10.1006/bbrc.1995.1824
Language
- eng
Conference Location
- United States