Nitric oxide produced by human B lymphocytes inhibits apoptosis and Epstein-Barr virus reactivation.

Journal Article

Nitric oxide (NO) produced by murine macrophages is important in murine resistance to ectromelia virus, herpes simplex virus, and vaccinia virus infection. In contrast, NO production by human mononuclear cells has been difficult to demonstrate, and a role for NO in human responses to infection is uncertain. We report constitutive, low level, macrophage-type NO synthase (iNOS) expression in Epstein-Barr virus (EBV)-transformed human B lymphocytes and Burkitt's lymphoma cell lines. Immune NOS activity is involved in maintaining EBV latency through down-regulation of the expression of the immediate-early EBV transactivator Zta. NO also inhibits apoptosis in B lymphocyte cell lines. The effects of NO are largely independent of cGMP and influential on signaling pathways regulated by (sulfhydryl) redox status. These results suggest that NO plays a physiological role in human B cell biology by inhibiting programmed cell death and maintaining viral latency.

Full Text

Duke Authors

Cited Authors

  • Mannick, JB; Asano, K; Izumi, K; Kieff, E; Stamler, JS

Published Date

  • December 30, 1994

Published In

Volume / Issue

  • 79 / 7

Start / End Page

  • 1137 - 1146

PubMed ID

  • 7528106

International Standard Serial Number (ISSN)

  • 0092-8674

Language

  • eng

Conference Location

  • United States