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beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling.

Publication ,  Journal Article
Hirata, F; Strittmatter, WJ; Axelrod, J
Published in: Proc Natl Acad Sci U S A
January 1979

The beta-adrenergic agonist L-isoproterenol stimulated the enzymic synthesis of phosphatidyl-N-monomethylethanolamine and phosphatidylcholine in rat reticulocyte ghosts containing the methyl donor S-adenosyl-L-methionine. The stimulation was stereospecific, dose-dependent, and inhibited by the beta-adrenergic agonist propranolol. The addition of GTP inside the resealed ghosts shifted the dose-response of phospholipid methylation by L-isoproterenol to the left by 2 orders of magnitude. Direct stimulation of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] with sodium fluoride or cholera toxin did not increase the methylation of phospholipids. At a concentration of S-adenosyl-L-methionine that stimulates synthesis of phosphatidyl-N-monomethylethanolamine, the activity of isoproterenol-sensitive adenylate cyclase was increased 2-fold without changes in the basal activity of adenylate cyclase and the number of beta-adrenergic receptors. The increase of phospholipid methylation by L-isoproterenol decreased membrane viscosity and increased translocation of methylated lipids. These findings indicate that enhancement of phospholipid methylation by L-isoproterenol decreases membrane microviscosity and thus increases lateral movement of the beta-adrenergic receptors and coupling with adenylate cyclase.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

January 1979

Volume

76

Issue

1

Start / End Page

368 / 372

Location

United States

Related Subject Headings

  • Reticulocytes
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats
  • Protein Binding
  • Phospholipids
  • Methylation
  • Membrane Lipids
  • Membrane Fluidity
  • Male
 

Citation

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Hirata, F., Strittmatter, W. J., & Axelrod, J. (1979). beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling. Proc Natl Acad Sci U S A, 76(1), 368–372. https://doi.org/10.1073/pnas.76.1.368
Hirata, F., W. J. Strittmatter, and J. Axelrod. “beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling.Proc Natl Acad Sci U S A 76, no. 1 (January 1979): 368–72. https://doi.org/10.1073/pnas.76.1.368.
Hirata, F., et al. “beta-Adrenergic receptor agonists increase phospholipid methylation, membrane fluidity, and beta-adrenergic receptor-adenylate cyclase coupling.Proc Natl Acad Sci U S A, vol. 76, no. 1, Jan. 1979, pp. 368–72. Pubmed, doi:10.1073/pnas.76.1.368.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

January 1979

Volume

76

Issue

1

Start / End Page

368 / 372

Location

United States

Related Subject Headings

  • Reticulocytes
  • Receptors, Adrenergic, beta
  • Receptors, Adrenergic
  • Rats
  • Protein Binding
  • Phospholipids
  • Methylation
  • Membrane Lipids
  • Membrane Fluidity
  • Male