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Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells.

Publication ,  Journal Article
Barber, KM; Pinero, A; Truskey, GA
Published in: The American journal of physiology
August 1998

We used a sudden-expansion flow chamber to examine U-937 cell adhesion to unactivated and tumor necrosis factor (TNF)-alpha-activated human umbilical vein endothelial cells (HUVEC) in recirculating flow. For both unactivated and TNF-alpha-activated HUVEC, U-937 cells exhibited transient arrests within approximately 150 microm of flow reattachment. Few arrests occurred directly at the reattachment site. U-937 cell rolling was not observed. At all other locations within the recirculation zone, U-937 cells did not exhibit transient arrests or rolling. TNF-alpha activation increased the frequency of U-937 cell arrests near reattachment but did not change the median arrest duration. Numerically simulated cell trajectories failed to predict attachment near the reattachment point. Deviations between experiment and theory may result from the nonspherical shape and deformability of U-937 cells. These results demonstrate that U-937 cell transient arrests occur preferentially in the vicinity of the reattachment point in recirculating flow. Possible mechanisms for adhesion include low shear stress, curved streamlines, fluid velocity components normal to the endothelium, and formation of larger contact areas.

Duke Scholars

Published In

The American journal of physiology

DOI

EISSN

2163-5773

ISSN

0002-9513

Publication Date

August 1998

Volume

275

Issue

2

Start / End Page

H591 / H599

Related Subject Headings

  • Umbilical Veins
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Time Factors
  • Stress, Mechanical
  • Models, Biological
  • Kinetics
  • Humans
  • Endothelium, Vascular
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
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Barber, K. M., Pinero, A., & Truskey, G. A. (1998). Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells. The American Journal of Physiology, 275(2), H591–H599. https://doi.org/10.1152/ajpheart.1998.275.2.h591
Barber, K. M., A. Pinero, and G. A. Truskey. “Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells.The American Journal of Physiology 275, no. 2 (August 1998): H591–99. https://doi.org/10.1152/ajpheart.1998.275.2.h591.
Barber KM, Pinero A, Truskey GA. Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells. The American journal of physiology. 1998 Aug;275(2):H591–9.
Barber, K. M., et al. “Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells.The American Journal of Physiology, vol. 275, no. 2, Aug. 1998, pp. H591–99. Epmc, doi:10.1152/ajpheart.1998.275.2.h591.
Barber KM, Pinero A, Truskey GA. Effects of recirculating flow on U-937 cell adhesion to human umbilical vein endothelial cells. The American journal of physiology. 1998 Aug;275(2):H591–H599.

Published In

The American journal of physiology

DOI

EISSN

2163-5773

ISSN

0002-9513

Publication Date

August 1998

Volume

275

Issue

2

Start / End Page

H591 / H599

Related Subject Headings

  • Umbilical Veins
  • Tumor Necrosis Factor-alpha
  • Tumor Cells, Cultured
  • Time Factors
  • Stress, Mechanical
  • Models, Biological
  • Kinetics
  • Humans
  • Endothelium, Vascular
  • Cells, Cultured