The filamentous bacteriophage assembly proteins require the bacterial SecA protein for correct localization to the membrane.

Journal Article (Journal Article)

The noncapsid assembly proteins pI and pI of the filamentous bacteriophage f1 are inserted into the inner membrane of Escherichia coli via an internal signal sequence. Inhibition of the activity of SecA with low concentrations of sodium azide results in rapid accumulation of pI and pI proteins in the cytoplasm. However, both proteins are inserted into the membrane under the same conditions when synthesized in bacteria containing a secA azide resistance mutation. The other noncapsid assembly protein, pIV, is an outer membrane protein synthesized with a cleavable signal sequence. Wild-type bacteria accumulate the precursor to pIV when protein synthesis is in the presence of low concentrations of sodium azide. These results suggest that the f1 bacteriophage assembly proteins require SecA and consequently the bacterial Sec system to reach their proper membrane location.

Full Text

Duke Authors

Cited Authors

  • Rapoza, MP; Webster, RE

Published Date

  • March 1, 1993

Published In

Volume / Issue

  • 175 / 6

Start / End Page

  • 1856 - 1859

PubMed ID

  • 8449893

Pubmed Central ID

  • PMC203996

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.175.6.1856-1859.1993


  • eng

Conference Location

  • United States