Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy.

Journal Article (Journal Article;Review)

The spatiotemporal localization of neuronal signaling is important for triggering neuronal responses in specific locations at precise times. Fluorescence resonance energy transfer imaging enables measurement of spatiotemporal dynamics of signaling activity in live neurons. Although the usefulness of fluorescence resonance energy transfer is well recognized, there are many difficulties in applying it, particularly when imaging in neuronal micro-compartments in light-scattering brain tissue. Fluorescence resonance energy transfer has been imaged using several techniques including intensity-based methods, fluorescence lifetime imaging and fluorescence anisotropy imaging. These methods have different advantages and disadvantages, and thus are suitable in different applications.

Full Text

Duke Authors

Cited Authors

  • Yasuda, R

Published Date

  • October 2006

Published In

Volume / Issue

  • 16 / 5

Start / End Page

  • 551 - 561

PubMed ID

  • 16971112

International Standard Serial Number (ISSN)

  • 0959-4388

Digital Object Identifier (DOI)

  • 10.1016/j.conb.2006.08.012


  • eng

Conference Location

  • England