Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors.

Published

Journal Article

Desmoid tumors (aggressive fibromatosis) are locally invasive soft tissue tumors in which beta-catenin-mediated TCF-3-dependent transcription is activated. To provide more insight into the pathophysiology of these tumors, expression profiles were generated using oligonucleotide arrays (Affymetrix). In total, 69 differentially expressed genes were identified in desmoids compared to normal fibroblasts (fascia) from the same patients. The differential expression of a selection of genes was confirmed using RT-PCR and Northern blotting. We further evaluated the insulin-like growth factor-binding protein 6 (IGFBP-6), a gene that was consistently downregulated in all desmoids tested. Promotor studies and electromobility shift assays revealed two functional beta-catenin/TCF-responsive elements in the human IGFBP-6 promoter. These findings suggest that IGFBP-6 is directly downregulated by the beta-catenin/TCF complex in desmoid tumors, and imply a role for the IGF axis in the proliferation of desmoid tumors.

Full Text

Duke Authors

Cited Authors

  • Denys, H; Jadidizadeh, A; Amini Nik, S; Van Dam, K; Aerts, S; Alman, BA; Cassiman, J-J; Tejpar, S

Published Date

  • January 2004

Published In

Volume / Issue

  • 23 / 3

Start / End Page

  • 654 - 664

PubMed ID

  • 14737101

Pubmed Central ID

  • 14737101

Electronic International Standard Serial Number (EISSN)

  • 1476-5594

International Standard Serial Number (ISSN)

  • 0950-9232

Digital Object Identifier (DOI)

  • 10.1038/sj.onc.1207160

Language

  • eng