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Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors.

Publication ,  Journal Article
Denys, H; Jadidizadeh, A; Amini Nik, S; Van Dam, K; Aerts, S; Alman, BA; Cassiman, J-J; Tejpar, S
Published in: Oncogene
January 22, 2004

Desmoid tumors (aggressive fibromatosis) are locally invasive soft tissue tumors in which beta-catenin-mediated TCF-3-dependent transcription is activated. To provide more insight into the pathophysiology of these tumors, expression profiles were generated using oligonucleotide arrays (Affymetrix). In total, 69 differentially expressed genes were identified in desmoids compared to normal fibroblasts (fascia) from the same patients. The differential expression of a selection of genes was confirmed using RT-PCR and Northern blotting. We further evaluated the insulin-like growth factor-binding protein 6 (IGFBP-6), a gene that was consistently downregulated in all desmoids tested. Promotor studies and electromobility shift assays revealed two functional beta-catenin/TCF-responsive elements in the human IGFBP-6 promoter. These findings suggest that IGFBP-6 is directly downregulated by the beta-catenin/TCF complex in desmoid tumors, and imply a role for the IGF axis in the proliferation of desmoid tumors.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

January 22, 2004

Volume

23

Issue

3

Start / End Page

654 / 664

Location

England

Related Subject Headings

  • beta Catenin
  • Tumor Cells, Cultured
  • Trans-Activators
  • Reverse Transcriptase Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Insulin-Like Growth Factor Binding Protein 6
  • Gene Expression Profiling
  • Fibroma
  • Down-Regulation
 

Citation

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Denys, H., Jadidizadeh, A., Amini Nik, S., Van Dam, K., Aerts, S., Alman, B. A., … Tejpar, S. (2004). Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors. Oncogene, 23(3), 654–664. https://doi.org/10.1038/sj.onc.1207160
Denys, Hannelore, Ali Jadidizadeh, Saeid Amini Nik, Kim Van Dam, Stein Aerts, Benjamin A. Alman, Jean-Jacques Cassiman, and Sabine Tejpar. “Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors.Oncogene 23, no. 3 (January 22, 2004): 654–64. https://doi.org/10.1038/sj.onc.1207160.
Denys H, Jadidizadeh A, Amini Nik S, Van Dam K, Aerts S, Alman BA, et al. Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors. Oncogene. 2004 Jan 22;23(3):654–64.
Denys, Hannelore, et al. “Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors.Oncogene, vol. 23, no. 3, Jan. 2004, pp. 654–64. Pubmed, doi:10.1038/sj.onc.1207160.
Denys H, Jadidizadeh A, Amini Nik S, Van Dam K, Aerts S, Alman BA, Cassiman J-J, Tejpar S. Identification of IGFBP-6 as a significantly downregulated gene by beta-catenin in desmoid tumors. Oncogene. 2004 Jan 22;23(3):654–664.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

January 22, 2004

Volume

23

Issue

3

Start / End Page

654 / 664

Location

England

Related Subject Headings

  • beta Catenin
  • Tumor Cells, Cultured
  • Trans-Activators
  • Reverse Transcriptase Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Oncology & Carcinogenesis
  • Insulin-Like Growth Factor Binding Protein 6
  • Gene Expression Profiling
  • Fibroma
  • Down-Regulation