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Agglutination of Naegleria fowleri and Naegleria gruberi by antibodies in human serum.

Publication ,  Journal Article
Reilly, MF; Marciano-Cabral, F; Bradley, DW; Bradley, SG
Published in: J Clin Microbiol
April 1983

The capability of serum samples from 423 human subjects to agglutinate rounded cells of Naegleria fowleri nN68 was assessed. Sera from the umbilical cords of seven infants failed to agglutinate N. fowleri cells. The median agglutination titer was 1:4 for sera from children through age 4 years, 1:8 for sera from juveniles 5 to 15 years of age, and 1:16 for sera from subjects 15 to 30 years old. The agglutination titers of sera from older adults decreased to a median of 1:8 for the 40- to 60-year-old age group and to 1:4 for the 60- to 90-year-old subjects. Serum samples from young adults agglutinated rounded cells of both N. fowleri and N. gruberi. The agglutination activity for N. fowleri was removed by absorption with N. fowleri but not with N. gruberi. Conversely, agglutination activity for N. gruberi was removed by absorption with N. gruberi but not with N. fowleri. The agglutinating activity for N. fowleri was immunoglobulin M. Serum samples from children displayed markedly disparate capabilities to agglutinate N. fowleri and N. gruberi. Only rounded cells of N. fowleri or N. gruberi were reliably agglutinated by human serum samples. Live or paraformaldehyde-killed cells could be used in the assay, but live N. gruberi cells returned to the amoeboid form, and these agglutinated poorly.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1983

Volume

17

Issue

4

Start / End Page

576 / 581

Location

United States

Related Subject Headings

  • Species Specificity
  • Middle Aged
  • Microbiology
  • Male
  • Infant, Newborn
  • Infant
  • Immunoglobulin M
  • Immunoglobulin G
  • Immunoglobulin A
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Reilly, M. F., Marciano-Cabral, F., Bradley, D. W., & Bradley, S. G. (1983). Agglutination of Naegleria fowleri and Naegleria gruberi by antibodies in human serum. J Clin Microbiol, 17(4), 576–581. https://doi.org/10.1128/jcm.17.4.576-581.1983
Reilly, M. F., F. Marciano-Cabral, D. W. Bradley, and S. G. Bradley. “Agglutination of Naegleria fowleri and Naegleria gruberi by antibodies in human serum.J Clin Microbiol 17, no. 4 (April 1983): 576–81. https://doi.org/10.1128/jcm.17.4.576-581.1983.
Reilly MF, Marciano-Cabral F, Bradley DW, Bradley SG. Agglutination of Naegleria fowleri and Naegleria gruberi by antibodies in human serum. J Clin Microbiol. 1983 Apr;17(4):576–81.
Reilly, M. F., et al. “Agglutination of Naegleria fowleri and Naegleria gruberi by antibodies in human serum.J Clin Microbiol, vol. 17, no. 4, Apr. 1983, pp. 576–81. Pubmed, doi:10.1128/jcm.17.4.576-581.1983.
Reilly MF, Marciano-Cabral F, Bradley DW, Bradley SG. Agglutination of Naegleria fowleri and Naegleria gruberi by antibodies in human serum. J Clin Microbiol. 1983 Apr;17(4):576–581.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1983

Volume

17

Issue

4

Start / End Page

576 / 581

Location

United States

Related Subject Headings

  • Species Specificity
  • Middle Aged
  • Microbiology
  • Male
  • Infant, Newborn
  • Infant
  • Immunoglobulin M
  • Immunoglobulin G
  • Immunoglobulin A
  • Humans