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Randomized, Double-Blind, Placebo-Controlled Trial of the Effect of Vitamin D3 on the Interferon Signature in Patients With Systemic Lupus Erythematosus.

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Aranow, C; Kamen, DL; Dall'Era, M; Massarotti, EM; Mackay, MC; Koumpouras, F; Coca, A; Chatham, WW; Clowse, MEB; Criscione-Schreiber, LG ...
Published in: Arthritis Rheumatol
July 2015

OBJECTIVE: Vitamin D modulates the immune response and blocks induction of an interferon (IFN) signature by systemic lupus erythematosus (SLE) sera. This study was undertaken to investigate the effects of vitamin D supplementation on the IFN signature in patients with SLE. METHODS: SLE patients (n = 57) with stable, inactive disease, a serum 25-hydroxyvitamin D (25[OH]D) level ≤20 ng/ml, an elevated anti-double-stranded DNA antibody level, and an IFN signature (as determined by measuring the expression levels of 3 IFN response genes) were randomized into a 12-week double-blind, placebo-controlled trial of vitamin D3 at doses of 2,000 IU or 4,000 IU. An IFN signature response was defined as a 50% reduction in the expression of 1 of the 3 genes or a 25% reduction in the expression of 2 of the 3 genes. Disease activity, adverse events, and endocrine effects were assessed. RESULTS: Baseline characteristics of the patients in the 3 treatment groups (placebo, low-dose vitamin D3 , or high-dose vitamin D3 ) were similar. Repletion of 25(OH)D (i.e., levels ≥30 ng/ml) was not observed in any of the patients who were receiving placebo, while repletion was observed in 16 of 33 patients receiving vitamin D3 . The percentage of patients with an IFN signature response did not differ among the treatment groups. Moreover, there was no difference in the percentage of patients with an IFN signature response between those who remained vitamin D deficient and those who demonstrated repletion of vitamin D. Modular microarray analysis of a subset of patients (n = 40) did not reveal changes from baseline in any modules (including the IFN-inducible module) in any of the treatment groups, and no differences in expression were found between patients who demonstrated vitamin D repletion and patients who were persistently vitamin D deficient. Vitamin D3 was well tolerated, and there were no safety concerns. CONCLUSION: Vitamin D3 supplementation up to 4,000 IU daily was safe and well-tolerated but failed to diminish the IFN signature in vitamin D-deficient SLE patients. Higher 25(OH)D levels sustained for a longer duration may be required to affect immunologic outcomes.

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Published In

Arthritis Rheumatol

DOI

EISSN

2326-5205

Publication Date

July 2015

Volume

67

Issue

7

Start / End Page

1848 / 1857

Location

United States

Related Subject Headings

  • Vitamin D
  • RNA-Binding Proteins
  • Prospective Studies
  • Myxovirus Resistance Proteins
  • Middle Aged
  • Microarray Analysis
  • Male
  • Lupus Erythematosus, Systemic
  • Humans
  • Gene Expression Regulation
 

Citation

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Aranow, C., Kamen, D. L., Dall’Era, M., Massarotti, E. M., Mackay, M. C., Koumpouras, F., … Diamond, B. (2015). Randomized, Double-Blind, Placebo-Controlled Trial of the Effect of Vitamin D3 on the Interferon Signature in Patients With Systemic Lupus Erythematosus. In Arthritis Rheumatol (Vol. 67, pp. 1848–1857). United States. https://doi.org/10.1002/art.39108
Aranow, Cynthia, Diane L. Kamen, Maria Dall’Era, Elena M. Massarotti, Meggan C. Mackay, Fotios Koumpouras, Andreea Coca, et al. “Randomized, Double-Blind, Placebo-Controlled Trial of the Effect of Vitamin D3 on the Interferon Signature in Patients With Systemic Lupus Erythematosus.” In Arthritis Rheumatol, 67:1848–57, 2015. https://doi.org/10.1002/art.39108.
Aranow C, Kamen DL, Dall’Era M, Massarotti EM, Mackay MC, Koumpouras F, et al. Randomized, Double-Blind, Placebo-Controlled Trial of the Effect of Vitamin D3 on the Interferon Signature in Patients With Systemic Lupus Erythematosus. In: Arthritis Rheumatol. 2015. p. 1848–57.
Aranow, Cynthia, et al. “Randomized, Double-Blind, Placebo-Controlled Trial of the Effect of Vitamin D3 on the Interferon Signature in Patients With Systemic Lupus Erythematosus.Arthritis Rheumatol, vol. 67, no. 7, 2015, pp. 1848–57. Pubmed, doi:10.1002/art.39108.
Aranow C, Kamen DL, Dall’Era M, Massarotti EM, Mackay MC, Koumpouras F, Coca A, Chatham WW, Clowse MEB, Criscione-Schreiber LG, Callahan S, Goldmuntz EA, Keyes-Elstein L, Oswald M, Gregersen PK, Diamond B. Randomized, Double-Blind, Placebo-Controlled Trial of the Effect of Vitamin D3 on the Interferon Signature in Patients With Systemic Lupus Erythematosus. Arthritis Rheumatol. 2015. p. 1848–1857.
Journal cover image

Published In

Arthritis Rheumatol

DOI

EISSN

2326-5205

Publication Date

July 2015

Volume

67

Issue

7

Start / End Page

1848 / 1857

Location

United States

Related Subject Headings

  • Vitamin D
  • RNA-Binding Proteins
  • Prospective Studies
  • Myxovirus Resistance Proteins
  • Middle Aged
  • Microarray Analysis
  • Male
  • Lupus Erythematosus, Systemic
  • Humans
  • Gene Expression Regulation