Experimentally-induced alterations in the kinetics of erythrocyte sickling
Erythrocyte sickling was measured in a reaction-limited system and the effect of pH, 2,3-DPG, and alkylureas on the rate of sickling was studied. Cells were subjected to sudden deoxygenation with sodium dithionite in a rapid-reaction parallel plate flow channel and individually tracked in time on video-tape. The time at which 50% of the originally discoidal cells sickled (t50) was measured. AS and SC cells demonstrated progressively decreased sickling times when the pH was lowered from 7.4 to 6.8. In contrast, there was little effect on the t50 of SS cells, unless the cells were in hypotonic medium. Metabolically induced decreases in red cell 2,3-DPG markedly increased the sickling times of SS cells. Alkylureas increased the t50 of SS cells 50% at 0.1 molar concentration. At 0.01 molar concentration no effect on the rate of sickling was observed. Alterations in sickling kinetics may be used in studying the effect of physiologic compounds and drugs on the sickling process.
Zarkowsky, HS; Hochmuth, RM
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