Rapid and transient localization of the leader RNA of vesicular stomatitis virus in the nuclei of infected cells.

Journal Article (Journal Article)

The leader RNA transcript from the 3' end of the genome of vesicular stomatitis virus (VSV) has been detected in both the nucleus and cytoplasm of infected baby hamster kidney (BHK) cells. In the cytoplasm, leader RNA accumulated gradually throughout the infection to about 200 molecules per cell at 6 hr after infection. In the nucleus, however, there was a sharp and rapid increase in the concentration of leader RNA to approximately equal to 300 molecules per cell at about 2 hr after infection that decreased rapidly by 3 hr. This report presents evidence for nuclear localization of transcription products of a (-)-strand RNA virus other than influenza and supports the hypothesis that the leader RNA plays a role in the shutoff of host cell transcription.

Full Text

Duke Authors

Cited Authors

  • Kurilla, MG; Piwnica-Worms, H; Keene, JD

Published Date

  • September 1982

Published In

Volume / Issue

  • 79 / 17

Start / End Page

  • 5240 - 5244

PubMed ID

  • 6291035

Pubmed Central ID

  • PMC346871

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.79.17.5240


  • eng

Conference Location

  • United States