Potent suppression of HIV-1 replication in humans by T-20, a peptide inhibitor of gp41-mediated virus entry.

Published

Journal Article

T-20, a synthetic peptide corresponding to a region of the transmembrane subunit of the HIV-1 envelope protein, blocks cell fusion and viral entry at concentrations of less than 2 ng/ml in vitro. We administered intravenous T-20 (monotherapy) for 14 days to sixteen HIV-infected adults in four dose groups (3, 10, 30 and 100 mg twice daily). There were significant, dose-related declines in plasma HIV RNA in all subjects who received higher dose levels. All four subjects receiving 100 mg twice daily had a decline in plasma HIV RNA to less than 500 copies/ml, by bDNA assay. A sensitive RT-PCR assay (detection threshold 40 copies/ml) demonstrated that, although undetectable levels were not achieved in the 14-day dosing period, there was a 1.96 log10 median decline in plasma HIV RNA in these subjects. This study provides proof-of-concept that viral entry can be successfully blocked in vivo. Short-term administration of T-20 seems safe and provides potent inhibition of HIV replication comparable to anti-retroviral regimens approved at present.

Full Text

Duke Authors

Cited Authors

  • Kilby, JM; Hopkins, S; Venetta, TM; DiMassimo, B; Cloud, GA; Lee, JY; Alldredge, L; Hunter, E; Lambert, D; Bolognesi, D; Matthews, T; Johnson, MR; Nowak, MA; Shaw, GM; Saag, MS

Published Date

  • November 1998

Published In

Volume / Issue

  • 4 / 11

Start / End Page

  • 1302 - 1307

PubMed ID

  • 9809555

Pubmed Central ID

  • 9809555

International Standard Serial Number (ISSN)

  • 1078-8956

Digital Object Identifier (DOI)

  • 10.1038/3293

Language

  • eng

Conference Location

  • United States