Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells
A method for detection, quantification, and characterization of protease activities present in the insect cell/baculovirus protein expression system was demonstrated. Proteases were found of 39, 44, and 52 kDa molecular weight. Enzymatic inhibitors (antipain, chymostatin, EDTA, pepstatin A), cofactors (e.g., ATP, Mg2+, Ca2+), pH, and substrates (gelatin, β-galactosidase, bovine serum albumin) were varied to improve and characterize the activities.
Naggie, S; Hu, YC; Pulliam-Holoman, TR; Bentley, WE
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