Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells


Journal Article

A method for detection, quantification, and characterization of protease activities present in the insect cell/baculovirus protein expression system was demonstrated. Proteases were found of 39, 44, and 52 kDa molecular weight. Enzymatic inhibitors (antipain, chymostatin, EDTA, pepstatin A), cofactors (e.g., ATP, Mg2+, Ca2+), pH, and substrates (gelatin, β-galactosidase, bovine serum albumin) were varied to improve and characterize the activities.

Full Text

Duke Authors

Cited Authors

  • Naggie, S; Hu, YC; Pulliam-Holoman, TR; Bentley, WE

Published Date

  • January 1, 1997

Published In

Volume / Issue

  • 11 / 5

Start / End Page

  • 297 - 300

International Standard Serial Number (ISSN)

  • 0951-208X

Digital Object Identifier (DOI)

  • 10.1023/A:1018411210412

Citation Source

  • Scopus