Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells
Publication
, Journal Article
Naggie, S; Hu, YC; Pulliam-Holoman, TR; Bentley, WE
Published in: Biotechnology Techniques
January 1, 1997
A method for detection, quantification, and characterization of protease activities present in the insect cell/baculovirus protein expression system was demonstrated. Proteases were found of 39, 44, and 52 kDa molecular weight. Enzymatic inhibitors (antipain, chymostatin, EDTA, pepstatin A), cofactors (e.g., ATP, Mg2+, Ca2+), pH, and substrates (gelatin, β-galactosidase, bovine serum albumin) were varied to improve and characterize the activities.
Duke Scholars
Published In
Biotechnology Techniques
DOI
ISSN
0951-208X
Publication Date
January 1, 1997
Volume
11
Issue
5
Start / End Page
297 / 300
Related Subject Headings
- Biotechnology
- 40 Engineering
- 31 Biological sciences
- 10 Technology
- 09 Engineering
- 06 Biological Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Naggie, S., Hu, Y. C., Pulliam-Holoman, T. R., & Bentley, W. E. (1997). Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells. Biotechnology Techniques, 11(5), 297–300. https://doi.org/10.1023/A:1018411210412
Naggie, S., Y. C. Hu, T. R. Pulliam-Holoman, and W. E. Bentley. “Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells.” Biotechnology Techniques 11, no. 5 (January 1, 1997): 297–300. https://doi.org/10.1023/A:1018411210412.
Naggie S, Hu YC, Pulliam-Holoman TR, Bentley WE. Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells. Biotechnology Techniques. 1997 Jan 1;11(5):297–300.
Naggie, S., et al. “Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells.” Biotechnology Techniques, vol. 11, no. 5, Jan. 1997, pp. 297–300. Scopus, doi:10.1023/A:1018411210412.
Naggie S, Hu YC, Pulliam-Holoman TR, Bentley WE. Substrate (gelatin) gel electrophoretic method for analysis of protease activity in insect (Sf-9) cells. Biotechnology Techniques. 1997 Jan 1;11(5):297–300.
Published In
Biotechnology Techniques
DOI
ISSN
0951-208X
Publication Date
January 1, 1997
Volume
11
Issue
5
Start / End Page
297 / 300
Related Subject Headings
- Biotechnology
- 40 Engineering
- 31 Biological sciences
- 10 Technology
- 09 Engineering
- 06 Biological Sciences