Ethylene production from 1‐aminocyclopropane‐1‐carboxylic acid in vitro: A mechanism for explaining ethylene production by a cell‐free preparation from pea epicotyls


Journal Article

The generation of ethylene from 1‐aminocyclopropane‐1‐carboxylic acid (ACC) added to a cell‐free preparation from etiolated pea (Pisum sativum L. cv. Alaska) epicotyls was found not to be due to a specific ACC oxidase or to oxygen radicals. Rather, endogenously produced H2O and manganese ions are coupled in a reaction sequence which produces ethylene from ACC. In a model system, H2O and Mn2+ converted ACC to ethylene under conditions similar to those in the pea preparation. Ultrafiltration of the pea preparation inhibited ethylene production, but it could be reconstituted either by adding an H2O2‐generating system to the ultrafiltrate or Mn2+ to the retentate. H2O2‐generating systems could reconstitute ethylene formation in a heat‐inactivated cell‐free sample while the loss of ability to produce ethylene upon dialysis of the pea preparation correlated with the loss of Mn2+ from the sample. Studies using cell‐free preparations to investigate ethylene synthesis should take care to exclude the possible involvement of H2O2 and Mn2+. Copyright © 1986, Wiley Blackwell. All rights reserved

Full Text

Duke Authors

Cited Authors

  • Stegink, SJ; Siedow, JN

Published Date

  • January 1, 1986

Published In

Volume / Issue

  • 66 / 4

Start / End Page

  • 625 - 631

Electronic International Standard Serial Number (EISSN)

  • 1399-3054

International Standard Serial Number (ISSN)

  • 0031-9317

Digital Object Identifier (DOI)

  • 10.1111/j.1399-3054.1986.tb05590.x

Citation Source

  • Scopus