Evidence for formation of superoxide and formate radicals in Methanobacterium formicicum.

Journal Article (Journal Article)

Using spin labeling and spin trapping techniques in combination with electron paramagnetic resonance spectrometry, we have detected the formation of superoxide by whole cells of Methanobacterium formicicum under aerobic conditions in the presence and absence of sodium formate. Rates of superoxide generation have been estimated. The formation of additional free radical species, including formate, was observed. Production of these and other free radicals resulted in lipid peroxidation and concomitant cell damage.

Full Text

Duke Authors

Cited Authors

  • Barber, MJ; Rosen, GM; Siegel, LM; Rauckman, EJ

Published Date

  • March 1983

Published In

Volume / Issue

  • 153 / 3

Start / End Page

  • 1282 - 1286

PubMed ID

  • 6298181

Pubmed Central ID

  • PMC221774

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.153.3.1282-1286.1983

Language

  • eng

Conference Location

  • United States