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A guanine nucleotide regulatory protein controls polyphosphoinositide metabolism, Ca2+ mobilization, and cellular responses to chemoattractants in human monocytes.

Publication ,  Journal Article
Verghese, MW; Smith, CD; Charles, LA; Jakoi, L; Synderman, R
Published in: J Immunol
July 1, 1986

Previous studies demonstrated that oligopeptide chemoattractant receptors on PMN and macrophages exist in high and low affinity states which are interconvertible by guanosine di- and triphosphates. These observations suggest that guanine nucleotide regulatory (N) proteins play a role in phagocyte activation by chemotactic factors. The data presented here indicate that chemotactic factor receptors on monocytes utilize an N protein to activate phospholipase C and subsequent biologic responses by the cells. This conclusion is based on the findings that inactivation of an N protein of 41,000 m.w. by Bordetella pertussis toxin (PT) treatment abolishes monocyte responsiveness to chemoattractants but not to lectins, PMA, or the Ca2+ ionophore A23187. Treatment with PT inhibited IP3 production, Ca2+ mobilization, and cellular activation as assessed by chemotaxis and changes in forward light scattering in response to the chemoattractants by at least 80%. Therefore, a PT-sensitive N protein plays an important role in the activation of monocytes by chemoattractants.

Duke Scholars

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 1, 1986

Volume

137

Issue

1

Start / End Page

271 / 275

Location

United States

Related Subject Headings

  • Virulence Factors, Bordetella
  • Subcellular Fractions
  • Phosphatidylinositols
  • Pertussis Toxin
  • Monocytes
  • Immunology
  • Humans
  • GTP-Binding Proteins
  • Chemotaxis, Leukocyte
  • Chemotactic Factors
 

Citation

APA
Chicago
ICMJE
MLA
NLM

Published In

J Immunol

ISSN

0022-1767

Publication Date

July 1, 1986

Volume

137

Issue

1

Start / End Page

271 / 275

Location

United States

Related Subject Headings

  • Virulence Factors, Bordetella
  • Subcellular Fractions
  • Phosphatidylinositols
  • Pertussis Toxin
  • Monocytes
  • Immunology
  • Humans
  • GTP-Binding Proteins
  • Chemotaxis, Leukocyte
  • Chemotactic Factors