Analysis of bacteriophage T4 coat proteins by two‐dimensional electrophoresis and computerized densitometry

Journal Article (Journal Article)

We have designed treatment conditions which allow analysis of the coat proteins of bacteriophage T4D+ by high resolution two‐dimensional electrophoresis. The best results were obtained when the mature phage particles were subjected to hypertoniclysis and DNase treatment before solubilization with sodium dodecyl sulfate (SDS). Samples containing 250 ug protein gave the optimum resolution, producing 45 spots as visualized by Coomassie Blue staining. Computerized densitometry measurements showed that these proteins range in relative abundance from less than 0.22% to 11.8% of the total. Copyright © 1981 Verlag Chemie, GmbH

Full Text

Duke Authors

Cited Authors

  • Gersten, DM; Kurian, P; Ledly, G; Park, CM; Suhocki, PV

Published Date

  • January 1, 1981

Published In

Volume / Issue

  • 2 / 2

Start / End Page

  • 123 - 125

Electronic International Standard Serial Number (EISSN)

  • 1522-2683

International Standard Serial Number (ISSN)

  • 0173-0835

Digital Object Identifier (DOI)

  • 10.1002/elps.1150020210

Citation Source

  • Scopus