The identification of a second actin-binding region in spinophilin/neurabin II.
Journal Article (Journal Article)
Spinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons. Previously, the actin-binding domain (ABD) of spinophilin was localized to a domain between amino acids (aa) 1 and 154. In a mass spectrometry screen for spinophilin-binding proteins, we have identified an additional actin-binding region between aa 151 and 282. F-actin co-sedimentation and GST affinity chromotography experiments further substantiate this result. Phalloidin staining of Rat2 fibroblasts transiently expressing GFP-spinophilin deletion constructs indicates co-localization with a subset of actin. Regions of spinophilin that lack the revised ABD (aa 1-230) do not co-localize with phalloidin-labeled actin, suggesting that the actin-binding domain contributes to directing the subcellular distribution of spinophilin. Targeting experiments using primary hippocampal cultures indicate that only the first actin-binding site contributes to dendritic spine localization. The second ABD targets to spines inefficiently and thus may interact with and affect actin filaments in a different manner than the first ABD.
Full Text
Duke Authors
Cited Authors
- Barnes, AP; Smith, FD; VanDongen, HM; VanDongen, AMJ; Milgram, SL
Published Date
- May 19, 2004
Published In
Volume / Issue
- 124 / 2
Start / End Page
- 105 - 113
PubMed ID
- 15135218
International Standard Serial Number (ISSN)
- 0169-328X
Digital Object Identifier (DOI)
- 10.1016/j.molbrainres.2003.12.020
Language
- eng
Conference Location
- Netherlands