A Mast Cell Degranulation Screening Assay for the Identification of Novel Mast Cell Activating Agents.

Published

Journal Article

The development and use of vaccines and their ability to prevent infection/disease is a shining example of the benefit of biomedical research. Modern vaccines often utilize subunit immunogens that exhibit minimal immunogenicity and require the use of adjuvants to maximize the induction of protective immune responses. We recently described a novel class of vaccine adjuvants, mast cell (MC) activators, that exhibit safe and effective vaccine adjuvant activity when administered by intranasal or intradermal routes. A compound library containing 580 functionalized benzopyrans, a structural motif found in a diverse array of natural and designed bioactive compounds, was screened using a MC degranulation assay to identify novel MC activating compounds for future evaluation as novel vaccine adjuvants. This approach identified 12 novel MC degranulating compounds. Therefore, MC degranulation can be used to reliably detect novel compounds for evaluation as adjuvants for use in mucosal vaccine strategies.

Full Text

Duke Authors

Cited Authors

  • Staats, HF; Kirwan, SM; Choi, HW; Shelburne, CP; Abraham, SN; Leung, GYC; Chen, DY-K

Published Date

  • January 1, 2013

Published In

Volume / Issue

  • 4 / 1

PubMed ID

  • 23667736

Pubmed Central ID

  • 23667736

International Standard Serial Number (ISSN)

  • 2040-2503

Digital Object Identifier (DOI)

  • 10.1039/C2MD20073B

Language

  • eng

Conference Location

  • England