An HMGA2-IGF2BP2 axis regulates myoblast proliferation and myogenesis.

Published

Journal Article

A group of genes that are highly and specifically expressed in proliferating skeletal myoblasts during myogenesis was identified. Expression of one of these genes, Hmga2, increases coincident with satellite cell activation, and later its expression significantly declines correlating with fusion of myoblasts into myotubes. Hmga2 knockout mice exhibit impaired muscle development and reduced myoblast proliferation, while overexpression of HMGA2 promotes myoblast growth. This perturbation in proliferation can be explained by the finding that HMGA2 directly regulates the RNA-binding protein IGF2BP2. Add-back of IGF2BP2 rescues the phenotype. IGF2BP2 in turn binds to and controls the translation of a set of mRNAs, including c-myc, Sp1, and Igf1r. These data demonstrate that the HMGA2-IGF2BP2 axis functions as a key regulator of satellite cell activation and therefore skeletal muscle development.

Full Text

Duke Authors

Cited Authors

  • Li, Z; Gilbert, JA; Zhang, Y; Zhang, M; Qiu, Q; Ramanujan, K; Shavlakadze, T; Eash, JK; Scaramozza, A; Goddeeris, MM; Kirsch, DG; Campbell, KP; Brack, AS; Glass, DJ

Published Date

  • December 2012

Published In

Volume / Issue

  • 23 / 6

Start / End Page

  • 1176 - 1188

PubMed ID

  • 23177649

Pubmed Central ID

  • 23177649

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2012.10.019

Language

  • eng