Beta-adrenergic receptor kinase-2 and beta-arrestin-2 as mediators of odorant-induced desensitization.
Journal Article (Journal Article)
beta-Adrenergic receptor kinase (beta ARK) and beta-arrestin function in the homologous or agonist-activated desensitization of G protein-coupled receptors. The isoforms beta ARK-2 and beta-arrestin-2 are highly enriched in and localized to the dendritic knobs and cilia of the olfactory receptor neurons where the initial events of olfactory signal transduction occur. Odorants induce a rapid and transient elevation of adenosine 3',5'-monophosphate (cAMP), which activates a nonspecific cation channel and produces membrane depolarization. Preincubation of rat olfactory cilia with antibodies raised against beta ARK-2 and beta-arrestin-2 increased the odorant-induced elevation of cAMP and attenuated desensitization. These results suggest that beta ARK-2 and beta-arrestin-2 mediate agonist-dependent desensitization in olfaction.
Full Text
Duke Authors
Cited Authors
- Dawson, TM; Arriza, JL; Jaworsky, DE; Borisy, FF; Attramadal, H; Lefkowitz, RJ; Ronnett, GV
Published Date
- February 5, 1993
Published In
Volume / Issue
- 259 / 5096
Start / End Page
- 825 - 829
PubMed ID
- 8381559
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.8381559
Language
- eng
Conference Location
- United States