Beta-adrenergic receptor kinase-2 and beta-arrestin-2 as mediators of odorant-induced desensitization.

Published

Journal Article

beta-Adrenergic receptor kinase (beta ARK) and beta-arrestin function in the homologous or agonist-activated desensitization of G protein-coupled receptors. The isoforms beta ARK-2 and beta-arrestin-2 are highly enriched in and localized to the dendritic knobs and cilia of the olfactory receptor neurons where the initial events of olfactory signal transduction occur. Odorants induce a rapid and transient elevation of adenosine 3',5'-monophosphate (cAMP), which activates a nonspecific cation channel and produces membrane depolarization. Preincubation of rat olfactory cilia with antibodies raised against beta ARK-2 and beta-arrestin-2 increased the odorant-induced elevation of cAMP and attenuated desensitization. These results suggest that beta ARK-2 and beta-arrestin-2 mediate agonist-dependent desensitization in olfaction.

Full Text

Duke Authors

Cited Authors

  • Dawson, TM; Arriza, JL; Jaworsky, DE; Borisy, FF; Attramadal, H; Lefkowitz, RJ; Ronnett, GV

Published Date

  • February 5, 1993

Published In

Volume / Issue

  • 259 / 5096

Start / End Page

  • 825 - 829

PubMed ID

  • 8381559

Pubmed Central ID

  • 8381559

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.8381559

Language

  • eng

Conference Location

  • United States