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Cell-free desensitization of catecholamine-sensitive adenylate cyclase. Agonist- and cAMP-promoted alterations in turkey erythrocyte beta-adrenergic receptors.

Publication ,  Journal Article
Nambi, P; Sibley, DR; Stadel, JM; Michel, T; Peters, JR; Lefkowitz, RJ
Published in: J Biol Chem
April 10, 1984

Conditions have been developed for desensitizing the beta-adrenergic receptor-coupled adenylate cyclase of turkey erythrocytes in a cell-free system. Desensitization is observed when cell lysates are incubated with isoproterenol or cAMP analogs for 30 min at 37 degrees C. Maximally effective concentrations of isoproterenol produce a 41.0 +/- 1.55% loss of iosproterenol-stimulated and a 15.0 +/- 2.35% loss of fluoride-stimulated enzyme activity. cAMP causes a 26.5 +/- 1.5% fall in isoproterenol-stimulated and a 21.5 +/- 4.4% fall in fluoride-sensitive activity. Desensitization by isoproterenol is dose-dependent, stereospecific, and blocked by the beta-adrenergic antagonist propranolol. Cell-free desensitization required ATP, Mg2+, and factor(s) present in the soluble fraction of the cell. Nonphosphorylating analogs of ATP did not support desensitization. Desensitization by agonist or cAMP in the cell-free system caused structural alterations in the beta-adrenergic receptor peptides apparent as an altered mobility of the photoaffinity labeled receptor peptides on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. As with the desensitization reaction, supernatant factors and ATP were also required for the agonist or cAMP-promoted receptor alterations. These data indicate that beta-adrenergic agonists promote a cAMP-mediated process which leads to receptor alterations and desensitization. The reactions involved in this process require ATP and soluble cellular factors. Additional processes must also occur to account for decreases in fluoride-sensitive enzyme activity. The availability of this cell-free system should facilitate elucidation of the molecular mechanisms involved in these processes.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 10, 1984

Volume

259

Issue

7

Start / End Page

4629 / 4633

Location

United States

Related Subject Headings

  • Turkeys
  • Receptors, Adrenergic, beta
  • Kinetics
  • Isoproterenol
  • Fluorides
  • Erythrocytes
  • Cyclic AMP
  • Bucladesine
  • Biochemistry & Molecular Biology
  • Animals
 

Citation

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Nambi, P., Sibley, D. R., Stadel, J. M., Michel, T., Peters, J. R., & Lefkowitz, R. J. (1984). Cell-free desensitization of catecholamine-sensitive adenylate cyclase. Agonist- and cAMP-promoted alterations in turkey erythrocyte beta-adrenergic receptors. J Biol Chem, 259(7), 4629–4633.
Nambi, P., D. R. Sibley, J. M. Stadel, T. Michel, J. R. Peters, and R. J. Lefkowitz. “Cell-free desensitization of catecholamine-sensitive adenylate cyclase. Agonist- and cAMP-promoted alterations in turkey erythrocyte beta-adrenergic receptors.J Biol Chem 259, no. 7 (April 10, 1984): 4629–33.
Nambi P, Sibley DR, Stadel JM, Michel T, Peters JR, Lefkowitz RJ. Cell-free desensitization of catecholamine-sensitive adenylate cyclase. Agonist- and cAMP-promoted alterations in turkey erythrocyte beta-adrenergic receptors. J Biol Chem. 1984 Apr 10;259(7):4629–33.
Nambi P, Sibley DR, Stadel JM, Michel T, Peters JR, Lefkowitz RJ. Cell-free desensitization of catecholamine-sensitive adenylate cyclase. Agonist- and cAMP-promoted alterations in turkey erythrocyte beta-adrenergic receptors. J Biol Chem. 1984 Apr 10;259(7):4629–4633.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 10, 1984

Volume

259

Issue

7

Start / End Page

4629 / 4633

Location

United States

Related Subject Headings

  • Turkeys
  • Receptors, Adrenergic, beta
  • Kinetics
  • Isoproterenol
  • Fluorides
  • Erythrocytes
  • Cyclic AMP
  • Bucladesine
  • Biochemistry & Molecular Biology
  • Animals