Effect of pertussis toxin on alpha 2-adrenoceptors: decreased formation of the high-affinity state for agonists.

Published

Journal Article

Administration of pertussis toxin to hamsters abolishes the alpha 2-adrenergic inhibition of adenylate cyclase in their adipocytes. The toxin did not modify the number of adipocyte alpha 2-adrenoceptors or their affinity for antagonists. In contrast, the affinity for agonists was significantly diminished in adipocyte membranes obtained from pertussis toxin-treated hamsters as compared to the controls. This decrease in affinity was due to a significant diminution in the proportion of sites displaying the high-affinity state for agonists. It is concluded that pertussis toxin induces a shift in the proportion of sites in high- and low-affinity states. for agonists towards the low-affinity conformation.

Full Text

Duke Authors

Cited Authors

  • García-Sáinz, JA; Boyer, JL; Michel, T; Sawyer, D; Stiles, GL; Dohlman, H; Lefkowitz, RJ

Published Date

  • June 25, 1984

Published In

Volume / Issue

  • 172 / 1

Start / End Page

  • 95 - 98

PubMed ID

  • 6329826

Pubmed Central ID

  • 6329826

International Standard Serial Number (ISSN)

  • 0014-5793

Language

  • eng

Conference Location

  • England