Synergistic and tunable human gene activation by combinations of synthetic transcription factors.

Journal Academic Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.

Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.

Full Text

Duke Authors

Cited Authors

  • Perez-Pinera, P; Ousterout, DG; Brunger, JM; Farin, AM; Glass, KA; Guilak, F; Crawford, GE; Hartemink, AJ; Gersbach, CA

Published Date

  • March 2013

Published In

Volume / Issue

  • 10 / 3

Start / End Page

  • 239 - 242

PubMed ID

  • 23377379

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

Digital Object Identifier (DOI)

  • 10.1038/nmeth.2361

Language

  • eng

Citation Source

  • PubMed