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Synergistic and tunable human gene activation by combinations of synthetic transcription factors.

Publication ,  Journal Article
Perez-Pinera, P; Ousterout, DG; Brunger, JM; Farin, AM; Glass, KA; Guilak, F; Crawford, GE; Hartemink, AJ; Gersbach, CA
Published in: Nat Methods
March 2013

Mammalian genes are regulated by the cooperative and synergistic actions of many transcription factors. In this study we recapitulate this complex regulation in human cells by targeting endogenous gene promoters, including regions of closed chromatin upstream of silenced genes, with combinations of engineered transcription activator-like effectors (TALEs). These combinations of TALE transcription factors induced substantial gene activation and allowed tuning of gene expression levels that will broadly enable synthetic biology, gene therapy and biotechnology.

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Published In

Nat Methods

DOI

EISSN

1548-7105

Publication Date

March 2013

Volume

10

Issue

3

Start / End Page

239 / 242

Location

United States

Related Subject Headings

  • Transfection
  • Transcriptional Activation
  • Transcription Factors
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Real-Time Polymerase Chain Reaction
  • Prostate-Specific Antigen
  • Promoter Regions, Genetic
  • Plasmids
  • Luciferases
 

Citation

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Perez-Pinera, P., Ousterout, D. G., Brunger, J. M., Farin, A. M., Glass, K. A., Guilak, F., … Gersbach, C. A. (2013). Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods, 10(3), 239–242. https://doi.org/10.1038/nmeth.2361
Perez-Pinera, Pablo, David G. Ousterout, Jonathan M. Brunger, Alicia M. Farin, Katherine A. Glass, Farshid Guilak, Gregory E. Crawford, Alexander J. Hartemink, and Charles A. Gersbach. “Synergistic and tunable human gene activation by combinations of synthetic transcription factors.Nat Methods 10, no. 3 (March 2013): 239–42. https://doi.org/10.1038/nmeth.2361.
Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, et al. Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods. 2013 Mar;10(3):239–42.
Perez-Pinera, Pablo, et al. “Synergistic and tunable human gene activation by combinations of synthetic transcription factors.Nat Methods, vol. 10, no. 3, Mar. 2013, pp. 239–42. Pubmed, doi:10.1038/nmeth.2361.
Perez-Pinera P, Ousterout DG, Brunger JM, Farin AM, Glass KA, Guilak F, Crawford GE, Hartemink AJ, Gersbach CA. Synergistic and tunable human gene activation by combinations of synthetic transcription factors. Nat Methods. 2013 Mar;10(3):239–242.

Published In

Nat Methods

DOI

EISSN

1548-7105

Publication Date

March 2013

Volume

10

Issue

3

Start / End Page

239 / 242

Location

United States

Related Subject Headings

  • Transfection
  • Transcriptional Activation
  • Transcription Factors
  • Receptor, erbB-2
  • Receptor, ErbB-2
  • Real-Time Polymerase Chain Reaction
  • Prostate-Specific Antigen
  • Promoter Regions, Genetic
  • Plasmids
  • Luciferases