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Controlled multicenter evaluation of a bacteriophage-based method for rapid detection of Staphylococcus aureus in positive blood cultures.

Publication ,  Journal Article
Bhowmick, T; Mirrett, S; Reller, LB; Price, C; Qi, C; Weinstein, MP; Kirn, TJ
Published in: J Clin Microbiol
April 2013

Staphylococci are a frequent cause of bloodstream infections (BSIs). Appropriate antibiotic treatment for BSIs may be delayed because conventional laboratory testing methods take 48 to 72 h to identify and characterize isolates from positive blood cultures. We evaluated a novel assay based on bacteriophage amplification that identifies Staphylococcus aureus and differentiates between methicillin-susceptible and methicillin-resistant S. aureus (MSSA and MRSA, respectively) in samples taken directly from signal-positive Bactec blood culture bottles within 24 h of positive signal, with results available within 5 h. The performance of the MicroPhage KeyPath MRSA/MSSA blood culture test was compared to conventional identification and susceptibility testing methods. At four sites, we collectively tested a total of 1,165 specimens, of which 1,116 were included in our analysis. Compared to standard methods, the KeyPath MRSA/MSSA blood culture test demonstrated a sensitivity, specificity, positive predictive value, and negative predictive value of 91.8%, 98.3%, 96.3%, and 96.1%, respectively, for correctly identifying S. aureus. Of those correctly identified as S. aureus (n = 334), 99.1% were correctly categorized as either MSSA or MRSA. Analysis of a subset of the data revealed that the KeyPath MRSA/MSSA blood culture test delivered results a median of 30 h sooner than conventional methods (a median of 46.9 h versus a median of 16.9 h). Although the sensitivity of the test in detecting S. aureus-positive samples is not high, its accuracy in determining methicillin resistance and susceptibility among positives is very high. These characteristics may enable earlier implementation of appropriate antibiotic treatment for many S. aureus BSI patients.

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Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

April 2013

Volume

51

Issue

4

Start / End Page

1226 / 1230

Location

United States

Related Subject Headings

  • Young Adult
  • Time Factors
  • Staphylococcus aureus
  • Staphylococcus Phages
  • Staphylococcal Infections
  • Sensitivity and Specificity
  • Predictive Value of Tests
  • Microbiology
  • Methicillin Resistance
  • Male
 

Citation

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Bhowmick, T., Mirrett, S., Reller, L. B., Price, C., Qi, C., Weinstein, M. P., & Kirn, T. J. (2013). Controlled multicenter evaluation of a bacteriophage-based method for rapid detection of Staphylococcus aureus in positive blood cultures. J Clin Microbiol, 51(4), 1226–1230. https://doi.org/10.1128/JCM.02967-12
Bhowmick, T., S. Mirrett, L. B. Reller, C. Price, C. Qi, M. P. Weinstein, and T. J. Kirn. “Controlled multicenter evaluation of a bacteriophage-based method for rapid detection of Staphylococcus aureus in positive blood cultures.J Clin Microbiol 51, no. 4 (April 2013): 1226–30. https://doi.org/10.1128/JCM.02967-12.
Bhowmick T, Mirrett S, Reller LB, Price C, Qi C, Weinstein MP, et al. Controlled multicenter evaluation of a bacteriophage-based method for rapid detection of Staphylococcus aureus in positive blood cultures. J Clin Microbiol. 2013 Apr;51(4):1226–30.
Bhowmick, T., et al. “Controlled multicenter evaluation of a bacteriophage-based method for rapid detection of Staphylococcus aureus in positive blood cultures.J Clin Microbiol, vol. 51, no. 4, Apr. 2013, pp. 1226–30. Pubmed, doi:10.1128/JCM.02967-12.
Bhowmick T, Mirrett S, Reller LB, Price C, Qi C, Weinstein MP, Kirn TJ. Controlled multicenter evaluation of a bacteriophage-based method for rapid detection of Staphylococcus aureus in positive blood cultures. J Clin Microbiol. 2013 Apr;51(4):1226–1230.

Published In

J Clin Microbiol

DOI

EISSN

1098-660X

Publication Date

April 2013

Volume

51

Issue

4

Start / End Page

1226 / 1230

Location

United States

Related Subject Headings

  • Young Adult
  • Time Factors
  • Staphylococcus aureus
  • Staphylococcus Phages
  • Staphylococcal Infections
  • Sensitivity and Specificity
  • Predictive Value of Tests
  • Microbiology
  • Methicillin Resistance
  • Male