Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus.
Published
Journal Article
Current human immunodeficiency virus-1 (HIV-1) vaccines elicit strain-specific neutralizing antibodies. However, cross-reactive neutralizing antibodies arise in approximately 20% of HIV-1-infected individuals, and details of their generation could provide a blueprint for effective vaccination. Here we report the isolation, evolution and structure of a broadly neutralizing antibody from an African donor followed from the time of infection. The mature antibody, CH103, neutralized approximately 55% of HIV-1 isolates, and its co-crystal structure with the HIV-1 envelope protein gp120 revealed a new loop-based mechanism of CD4-binding-site recognition. Virus and antibody gene sequencing revealed concomitant virus evolution and antibody maturation. Notably, the unmutated common ancestor of the CH103 lineage avidly bound the transmitted/founder HIV-1 envelope glycoprotein, and evolution of antibody neutralization breadth was preceded by extensive viral diversification in and near the CH103 epitope. These data determine the viral and antibody evolution leading to induction of a lineage of HIV-1 broadly neutralizing antibodies, and provide insights into strategies to elicit similar antibodies by vaccination.
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Duke Authors
- Alam, S. Munir
- Gao, Feng
- Haynes, Barton Ford
- Kelsoe, Garnett H.
- Liao, Hua-Xin
- Montefiori, David Charles
- Wiehe, Kevin J
Cited Authors
- Liao, H-X; Lynch, R; Zhou, T; Gao, F; Alam, SM; Boyd, SD; Fire, AZ; Roskin, KM; Schramm, CA; Zhang, Z; Zhu, J; Shapiro, L; NISC Comparative Sequencing Program, ; Mullikin, JC; Gnanakaran, S; Hraber, P; Wiehe, K; Kelsoe, G; Yang, G; Xia, S-M; Montefiori, DC; Parks, R; Lloyd, KE; Scearce, RM; Soderberg, KA; Cohen, M; Kamanga, G; Louder, MK; Tran, LM; Chen, Y; Cai, F; Chen, S; Moquin, S; Du, X; Joyce, MG; Srivatsan, S; Zhang, B; Zheng, A; Shaw, GM; Hahn, BH; Kepler, TB; Korber, BTM; Kwong, PD; Mascola, JR; Haynes, BF
Published Date
- April 25, 2013
Published In
Volume / Issue
- 496 / 7446
Start / End Page
- 469 - 476
PubMed ID
- 23552890
Pubmed Central ID
- 23552890
Electronic International Standard Serial Number (EISSN)
- 1476-4687
Digital Object Identifier (DOI)
- 10.1038/nature12053
Language
- eng
Conference Location
- England