A multimodal micro-optrode combining field and single unit recording, multispectral detection and photolabeling capabilities.

Published

Journal Article

Microelectrodes have been very instrumental and minimally invasive for in vivo functional studies from deep brain structures. However they are limited in the amount of information they provide. Here, we describe a, aluminum-coated, fibre optic-based glass microprobe with multiple electrical and optical detection capabilities while retaining tip dimensions that enable single cell measurements (diameter ≤10 µm). The probe enables optical separation from individual cells in transgenic mice expressing multiple fluorescent proteins in distinct populations of neurons within the same deep brain nucleus. It also enables color conversion of photoswitchable fluorescent proteins, which can be used for post-hoc identification of the recorded cells. While metal coating did not significantly improve the optical separation capabilities of the microprobe, the combination of metal on the outside of the probe and of a hollow core within the fiber yields a microelectrode enabling simultaneous single unit and population field potential recordings. The extended range of functionalities provided by the same microprobe thus opens several avenues for multidimensional structural and functional interrogation of single cells and their surrounding deep within the intact nervous system.

Full Text

Duke Authors

Cited Authors

  • Dufour, S; Lavertu, G; Dufour-Beauséjour, S; Juneau-Fecteau, A; Calakos, N; Deschênes, M; Vallée, R; De Koninck, Y

Published Date

  • January 2013

Published In

Volume / Issue

  • 8 / 2

Start / End Page

  • e57703 -

PubMed ID

  • 23469053

Pubmed Central ID

  • 23469053

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

International Standard Serial Number (ISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0057703

Language

  • eng