The role of single-cell analyses in understanding cell lineage commitment.

Journal Article (Review;Journal Article)

The study of cell lineage commitment is critical for improving our understanding of tissue development and regeneration, and for realizing stem cell-based therapies and engineered tissue replacements. Recently, the discovery of an unanticipated degree of variability in fundamental biological processes, including divergent responses of genetically identical cells to various stimuli, has provided mechanistic insight into cellular decision making and the collective behavior of cell populations. Therefore, the study of lineage commitment with single-cell resolution could provide greater knowledge of cellular differentiation mechanisms and the influence of noise on cellular processes. This will require the adoption of new technologies for single-cell analysis as traditional methods typically measure average values of bulk population behavior. This review discusses the recent developments in methods for analyzing the behavior of individual cells, and how these approaches are leading to a deeper understanding and better control of cellular decision making.

Full Text

Duke Authors

Cited Authors

  • Gibson, TM; Gersbach, CA

Published Date

  • April 2013

Published In

Volume / Issue

  • 8 / 4

Start / End Page

  • 397 - 407

PubMed ID

  • 23520130

Pubmed Central ID

  • PMC3753774

Electronic International Standard Serial Number (EISSN)

  • 1860-7314

International Standard Serial Number (ISSN)

  • 1860-6768

Digital Object Identifier (DOI)

  • 10.1002/biot.201200201


  • eng