The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile.
Journal Article (Journal Article)
We have examined the polar lipids of Clostridium psychrophilum, a recently characterized psychrophilic Clostridium isolated from an Antarctic microbial mat. Lipids were extracted from cells grown near the optimal growth temperature (+5°C) and at -5°C, and analyzed by two-dimensional thin layer chromatography and liquid chromatography coupled with mass spectrometry. The major phospholipids of this species are: cardiolipin, phosphatidylethanolamine, and phosphatidylglycerol. Phosphatidylserine and lyso-phosphatidylethanolamine were found as minor components. The most abundant glycolipids are a monoglycosyldiradylglycerol (MGDRG) and a diglycosyldiradylglycerol (DGDRG). The latter was only seen in cells grown at -5°C. An ethanolamine-phosphate derivative of N-acetylglucosaminyldiradylglycerol was seen in cells grown at -5°C and an ethanolamine-phosphate derivative of MGDRG was found in cells grown at +5°C. All lipids were present in both the all acyl and plasmalogen (alk-1'-enyl acyl) forms with the exception of PS and MGDRG, which were predominantly in the diacyl form. The significance of lipid changes at the two growth temperatures is discussed.
Full Text
Duke Authors
Cited Authors
- Guan, Z; Tian, B; Perfumo, A; Goldfine, H
Published Date
- June 2013
Published In
Volume / Issue
- 1831 / 6
Start / End Page
- 1108 - 1112
PubMed ID
- 23454375
Pubmed Central ID
- PMC3780410
International Standard Serial Number (ISSN)
- 0006-3002
Digital Object Identifier (DOI)
- 10.1016/j.bbalip.2013.02.004
Language
- eng
Conference Location
- Netherlands