The polar lipids of Clostridium psychrophilum, an anaerobic psychrophile.

Published

Journal Article

We have examined the polar lipids of Clostridium psychrophilum, a recently characterized psychrophilic Clostridium isolated from an Antarctic microbial mat. Lipids were extracted from cells grown near the optimal growth temperature (+5°C) and at -5°C, and analyzed by two-dimensional thin layer chromatography and liquid chromatography coupled with mass spectrometry. The major phospholipids of this species are: cardiolipin, phosphatidylethanolamine, and phosphatidylglycerol. Phosphatidylserine and lyso-phosphatidylethanolamine were found as minor components. The most abundant glycolipids are a monoglycosyldiradylglycerol (MGDRG) and a diglycosyldiradylglycerol (DGDRG). The latter was only seen in cells grown at -5°C. An ethanolamine-phosphate derivative of N-acetylglucosaminyldiradylglycerol was seen in cells grown at -5°C and an ethanolamine-phosphate derivative of MGDRG was found in cells grown at +5°C. All lipids were present in both the all acyl and plasmalogen (alk-1'-enyl acyl) forms with the exception of PS and MGDRG, which were predominantly in the diacyl form. The significance of lipid changes at the two growth temperatures is discussed.

Full Text

Duke Authors

Cited Authors

  • Guan, Z; Tian, B; Perfumo, A; Goldfine, H

Published Date

  • June 2013

Published In

Volume / Issue

  • 1831 / 6

Start / End Page

  • 1108 - 1112

PubMed ID

  • 23454375

Pubmed Central ID

  • 23454375

Electronic International Standard Serial Number (EISSN)

  • 1878-2434

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/j.bbalip.2013.02.004

Language

  • eng