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Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation.

Publication ,  Journal Article
McNulty, AL; Rothfusz, NE; Leddy, HA; Guilak, F
Published in: J Orthop Res
July 2013

Cartilage degeneration with osteoarthritis (OA) is believed to involve the activities of interleukin-1 (IL-1), which exists as alpha and beta isoforms. The goal of this study was to measure the concentrations of both isoforms of IL-1 in the synovial fluid of normal and spontaneously osteoarthritic porcine knees, and to test the hypothesis that physiologic concentrations of IL-1α and IL-1β exhibit different potencies in activating calcium signaling, the production of matrix metalloproteinases and nitric oxide, and the loss of proteoglycans and tissue mechanical properties in cartilage and meniscus. Median concentrations of IL-1α were 0.043 ng/ml with mild OA and 0.288 ng/ml with moderate OA, whereas IL-1β concentrations were 0.109 ng/ml with mild OA and 0.122 ng/ml with moderate OA. Both isoforms induced calcium signaling in chondrocytes and meniscal cells at all concentrations. Overall, cartilage and meniscus catabolism was significantly more sensitive to IL-1α than IL-1β at concentrations of 1 ng/ml or less, while few differences were observed between the two forms at 10 ng/ml. These data provide a range of physiologic IL-1 concentrations that can serve as a framework for the comparison of various in vitro studies, as well as providing further insight for the development of anti-cytokine therapies for OA.

Duke Scholars

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

July 2013

Volume

31

Issue

7

Start / End Page

1039 / 1045

Location

United States

Related Subject Headings

  • Synovial Fluid
  • Swine
  • Severity of Illness Index
  • Proteoglycans
  • Osteoarthritis, Knee
  • Orthopedics
  • Menisci, Tibial
  • Matrix Metalloproteinases
  • Interleukin-1beta
  • Interleukin-1alpha
 

Citation

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McNulty, A. L., Rothfusz, N. E., Leddy, H. A., & Guilak, F. (2013). Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation. J Orthop Res, 31(7), 1039–1045. https://doi.org/10.1002/jor.22334
McNulty, Amy L., Nicole E. Rothfusz, Holly A. Leddy, and Farshid Guilak. “Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation.J Orthop Res 31, no. 7 (July 2013): 1039–45. https://doi.org/10.1002/jor.22334.
McNulty AL, Rothfusz NE, Leddy HA, Guilak F. Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation. J Orthop Res. 2013 Jul;31(7):1039–45.
McNulty, Amy L., et al. “Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation.J Orthop Res, vol. 31, no. 7, July 2013, pp. 1039–45. Pubmed, doi:10.1002/jor.22334.
McNulty AL, Rothfusz NE, Leddy HA, Guilak F. Synovial fluid concentrations and relative potency of interleukin-1 alpha and beta in cartilage and meniscus degradation. J Orthop Res. 2013 Jul;31(7):1039–1045.
Journal cover image

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

July 2013

Volume

31

Issue

7

Start / End Page

1039 / 1045

Location

United States

Related Subject Headings

  • Synovial Fluid
  • Swine
  • Severity of Illness Index
  • Proteoglycans
  • Osteoarthritis, Knee
  • Orthopedics
  • Menisci, Tibial
  • Matrix Metalloproteinases
  • Interleukin-1beta
  • Interleukin-1alpha