Using DNase digestion data to accurately identify transcription factor binding sites.

Published

Journal Article

Identifying binding sites of transcription factors (TFs) is a key task in deciphering transcriptional regulation. ChIP-based methods are used to survey the genomic locations of a single TF in each experiment. But methods combining DNase digestion data with TF binding specificity information could potentially be used to survey the locations of many TFs in the same experiment, provided such methods permit reasonable levels of sensitivity and specificity. Here, we present a simple such method that outperforms a leading recent method, centipede, marginally in human but dramatically in yeast (average auROC across 20 TFs increases from 74% to 94%). Our method is based on logistic regression and thus benefits from supervision, but we show that partially and completely unsupervised variants perform nearly as well. Because the number of parameters in our method is at least an order of magnitude smaller than CENTIPEDE, we dub it MILLIPEDE.

Full Text

Duke Authors

Cited Authors

  • Luo, K; Hartemink, AJ

Published Date

  • January 2013

Published In

Start / End Page

  • 80 - 91

PubMed ID

  • 23424114

Pubmed Central ID

  • 23424114

Electronic International Standard Serial Number (EISSN)

  • 2335-6936

International Standard Serial Number (ISSN)

  • 2335-6928

Language

  • eng