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The utility of micro-CT and MRI in the assessment of longitudinal growth of liver metastases in a preclinical model of colon carcinoma.

Publication ,  Journal Article
Pandit, P; Johnston, SM; Qi, Y; Story, J; Nelson, R; Johnson, GA
Published in: Acad Radiol
April 2013

RATIONALE AND OBJECTIVES: Liver is a common site for distal metastases in colon and rectal cancer. Numerous clinical studies have analyzed the relative merits of different imaging modalities for detection of liver metastases. Several exciting new therapies are being investigated in preclinical models. But, technical challenges in preclinical imaging make it difficult to translate conclusions from clinical studies to the preclinical environment. This study addresses the technical challenges of preclinical magnetic resonance imaging (MRI) and micro-computed tomography (CT) to enable comparison of state-of-the-art methods for following metastatic liver disease. MATERIALS AND METHODS: We optimized two promising preclinical protocols to enable a parallel longitudinal study tracking metastatic human colon carcinoma growth in a mouse model: T2-weighted MRI using two-shot PROPELLER (Periodically Rotated Overlapping ParallEL Lines with Enhanced Reconstruction) and contrast-enhanced micro-CT using a liposomal contrast agent. Both methods were tailored for high throughput with attention to animal support and anesthesia to limit biological stress. RESULTS AND CONCLUSIONS: Each modality has its strengths. Micro-CT permitted more rapid acquisition (<10 minutes) with the highest spatial resolution (88-micron isotropic resolution). But detection of metastatic lesions requires the use of a blood pool contrast agent, which could introduce a confound in the evaluation of new therapies. MRI was slower (30 minutes) and had lower anisotropic spatial resolution. But MRI eliminates the need for a contrast agent and the contrast-to-noise between tumor and normal parenchyma was higher, making earlier detection of small lesions possible. Both methods supported a relatively high-throughput, longitudinal study of the development of metastatic lesions.

Duke Scholars

Published In

Acad Radiol

DOI

EISSN

1878-4046

Publication Date

April 2013

Volume

20

Issue

4

Start / End Page

430 / 439

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Nuclear Medicine & Medical Imaging
  • Microradiography
  • Mice, Nude
  • Mice
  • Magnetic Resonance Imaging
  • Liver Neoplasms
  • Image Processing, Computer-Assisted
  • Female
  • Contrast Media
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pandit, P., Johnston, S. M., Qi, Y., Story, J., Nelson, R., & Johnson, G. A. (2013). The utility of micro-CT and MRI in the assessment of longitudinal growth of liver metastases in a preclinical model of colon carcinoma. Acad Radiol, 20(4), 430–439. https://doi.org/10.1016/j.acra.2012.09.030
Pandit, Prachi, Samuel M. Johnston, Yi Qi, Jennifer Story, Rendon Nelson, and G Allan Johnson. “The utility of micro-CT and MRI in the assessment of longitudinal growth of liver metastases in a preclinical model of colon carcinoma.Acad Radiol 20, no. 4 (April 2013): 430–39. https://doi.org/10.1016/j.acra.2012.09.030.
Pandit P, Johnston SM, Qi Y, Story J, Nelson R, Johnson GA. The utility of micro-CT and MRI in the assessment of longitudinal growth of liver metastases in a preclinical model of colon carcinoma. Acad Radiol. 2013 Apr;20(4):430–9.
Pandit, Prachi, et al. “The utility of micro-CT and MRI in the assessment of longitudinal growth of liver metastases in a preclinical model of colon carcinoma.Acad Radiol, vol. 20, no. 4, Apr. 2013, pp. 430–39. Pubmed, doi:10.1016/j.acra.2012.09.030.
Pandit P, Johnston SM, Qi Y, Story J, Nelson R, Johnson GA. The utility of micro-CT and MRI in the assessment of longitudinal growth of liver metastases in a preclinical model of colon carcinoma. Acad Radiol. 2013 Apr;20(4):430–439.
Journal cover image

Published In

Acad Radiol

DOI

EISSN

1878-4046

Publication Date

April 2013

Volume

20

Issue

4

Start / End Page

430 / 439

Location

United States

Related Subject Headings

  • Tomography, X-Ray Computed
  • Nuclear Medicine & Medical Imaging
  • Microradiography
  • Mice, Nude
  • Mice
  • Magnetic Resonance Imaging
  • Liver Neoplasms
  • Image Processing, Computer-Assisted
  • Female
  • Contrast Media