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Immunogenicity of a vaccine regimen composed of simian immunodeficiency virus DNA, rMVA, and viral particles administered to female rhesus macaques via four different mucosal routes.

Publication ,  Journal Article
Manrique, M; Kozlowski, PA; Cobo-Molinos, A; Wang, S-W; Wilson, RL; Montefiori, DC; Carville, A; Aldovini, A
Published in: J Virol
April 2013

A comparative evaluation of the immunity stimulated with a vaccine regimen that includes simian immunodeficiency virus (SIV), interleukin 2 (IL-2), and IL-15 DNAs, recombinant modified vaccinia virus Ankara (rMVA), and inactivated SIVmac239 particles administered into the oral and nasal cavities, small intestine, and vagina was carried out in female rhesus macaques to determine the best route to induce diverse anti-SIV immunity that may be critical to protection from SIV infection and disease. All four immunizations generated mucosal SIV-specific IgA. Oral immunization was as effective as vaginal immunization in inducing SIV-specific IgA in vaginal secretions and generated greater IgA responses in rectal secretions and saliva samples compared to the other immunization routes. All four immunizations stimulated systemic T-cell responses against Gag and Env, albeit to a different extent, with oral immunization providing greater magnitude and nasal immunization providing wider functional heterogeneity. SIV-specific T cells producing gamma interferon (IFN-γ) dominated these responses. Limited levels of SIV-specific IgG antibodies were detected in plasma samples, and no SIV-specific IgG antibodies were detected in secretions. Vaccination also induced CD4(+) and CD8(+) T-cell responses in the rectal and vaginal mucosa with greater functional heterogeneity than in blood samples. Rectal T-cell responses were significantly greater in the orally vaccinated animals than in the other animals. The most balanced, diverse, and higher-magnitude vaginal T-cell responses were observed after intestinal vaccination. Significantly higher CD8(+) granzyme B-positive T-cell responses were observed systemically after intestinal vaccination and in rectal cells after oral immunization. The majority of SIV-specific T cells that produced granzyme B did not produce cytokines. Of the immunization routes tested, oral vaccination provided the most diverse and significant response to the vaccine.

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Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

April 2013

Volume

87

Issue

8

Start / End Page

4738 / 4750

Location

United States

Related Subject Headings

  • Virology
  • Vaccinia virus
  • Vaccines, Synthetic
  • Vaccines, Inactivated
  • Vaccines, DNA
  • Vaccination
  • T-Lymphocytes
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines
 

Citation

APA
Chicago
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MLA
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Manrique, M., Kozlowski, P. A., Cobo-Molinos, A., Wang, S.-W., Wilson, R. L., Montefiori, D. C., … Aldovini, A. (2013). Immunogenicity of a vaccine regimen composed of simian immunodeficiency virus DNA, rMVA, and viral particles administered to female rhesus macaques via four different mucosal routes. J Virol, 87(8), 4738–4750. https://doi.org/10.1128/JVI.03531-12
Manrique, Mariana, Pamela A. Kozlowski, Antonio Cobo-Molinos, Shainn-Wei Wang, Robert L. Wilson, David C. Montefiori, Angela Carville, and Anna Aldovini. “Immunogenicity of a vaccine regimen composed of simian immunodeficiency virus DNA, rMVA, and viral particles administered to female rhesus macaques via four different mucosal routes.J Virol 87, no. 8 (April 2013): 4738–50. https://doi.org/10.1128/JVI.03531-12.
Manrique M, Kozlowski PA, Cobo-Molinos A, Wang S-W, Wilson RL, Montefiori DC, Carville A, Aldovini A. Immunogenicity of a vaccine regimen composed of simian immunodeficiency virus DNA, rMVA, and viral particles administered to female rhesus macaques via four different mucosal routes. J Virol. 2013 Apr;87(8):4738–4750.

Published In

J Virol

DOI

EISSN

1098-5514

Publication Date

April 2013

Volume

87

Issue

8

Start / End Page

4738 / 4750

Location

United States

Related Subject Headings

  • Virology
  • Vaccinia virus
  • Vaccines, Synthetic
  • Vaccines, Inactivated
  • Vaccines, DNA
  • Vaccination
  • T-Lymphocytes
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • SAIDS Vaccines