Antibody-DEPENDENT, FcγRI-mediated neutralization of HIV-1 in TZM-bl cells occurs independently of phagocytosis.
Publication
, Journal Article
Perez, LG; Zolla-Pazner, S; Montefiori, DC
Published in: J Virol
May 2013
We previously showed that expression of human FcγRI on TZM-bl cells potentiates neutralization by gp41 membrane-proximal external region (MPER)-specific antibodies. Here we show that lysosomotropic reagents known to block phagocytosis do not diminish this effect. We also show that FcγRI occasionally potentiates neutralization by antibodies against the V3 loop of gp120 and cluster I of gp41. We conclude that FcγRI provides a kinetic advantage for neutralizing antibodies against partially cryptic epitopes independent of phagocytosis.
Duke Scholars
Published In
J Virol
DOI
EISSN
1098-5514
Publication Date
May 2013
Volume
87
Issue
9
Start / End Page
5287 / 5290
Location
United States
Related Subject Headings
- Virology
- Receptors, IgG
- Phagocytosis
- Neutralization Tests
- Humans
- HIV-1
- HIV Infections
- HIV Envelope Protein gp120
- HIV Antibodies
- Cell Line
Citation
APA
Chicago
ICMJE
MLA
NLM
Perez, L. G., Zolla-Pazner, S., & Montefiori, D. C. (2013). Antibody-DEPENDENT, FcγRI-mediated neutralization of HIV-1 in TZM-bl cells occurs independently of phagocytosis. J Virol, 87(9), 5287–5290. https://doi.org/10.1128/JVI.00278-13
Perez, Lautaro G., Susan Zolla-Pazner, and David C. Montefiori. “Antibody-DEPENDENT, FcγRI-mediated neutralization of HIV-1 in TZM-bl cells occurs independently of phagocytosis.” J Virol 87, no. 9 (May 2013): 5287–90. https://doi.org/10.1128/JVI.00278-13.
Perez LG, Zolla-Pazner S, Montefiori DC. Antibody-DEPENDENT, FcγRI-mediated neutralization of HIV-1 in TZM-bl cells occurs independently of phagocytosis. J Virol. 2013 May;87(9):5287–90.
Perez, Lautaro G., et al. “Antibody-DEPENDENT, FcγRI-mediated neutralization of HIV-1 in TZM-bl cells occurs independently of phagocytosis.” J Virol, vol. 87, no. 9, May 2013, pp. 5287–90. Pubmed, doi:10.1128/JVI.00278-13.
Perez LG, Zolla-Pazner S, Montefiori DC. Antibody-DEPENDENT, FcγRI-mediated neutralization of HIV-1 in TZM-bl cells occurs independently of phagocytosis. J Virol. 2013 May;87(9):5287–5290.
Published In
J Virol
DOI
EISSN
1098-5514
Publication Date
May 2013
Volume
87
Issue
9
Start / End Page
5287 / 5290
Location
United States
Related Subject Headings
- Virology
- Receptors, IgG
- Phagocytosis
- Neutralization Tests
- Humans
- HIV-1
- HIV Infections
- HIV Envelope Protein gp120
- HIV Antibodies
- Cell Line