Antibody-DEPENDENT, FcγRI-mediated neutralization of HIV-1 in TZM-bl cells occurs independently of phagocytosis.

Published

Journal Article

We previously showed that expression of human FcγRI on TZM-bl cells potentiates neutralization by gp41 membrane-proximal external region (MPER)-specific antibodies. Here we show that lysosomotropic reagents known to block phagocytosis do not diminish this effect. We also show that FcγRI occasionally potentiates neutralization by antibodies against the V3 loop of gp120 and cluster I of gp41. We conclude that FcγRI provides a kinetic advantage for neutralizing antibodies against partially cryptic epitopes independent of phagocytosis.

Full Text

Duke Authors

Cited Authors

  • Perez, LG; Zolla-Pazner, S; Montefiori, DC

Published Date

  • May 2013

Published In

Volume / Issue

  • 87 / 9

Start / End Page

  • 5287 - 5290

PubMed ID

  • 23408628

Pubmed Central ID

  • 23408628

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

Digital Object Identifier (DOI)

  • 10.1128/JVI.00278-13

Language

  • eng

Conference Location

  • United States